Alpha-tubulin Antibody

Blue Ice

Predicted: 50 kDa
Observed: 45 kDa

Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

Trial Size

17921993

PRS-1303-0.1mg

1 mg/mL

Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Alpha-tubulin antibody is human, mouse and rat reactive.

Polyclonal

homo sapiens

Optimal dilutions for each application to be determined by the researcher.