Rubicon Antibody

Blue Ice

Predicted: 90, 101, 109 kDa
Observed: 91, 99 kDa

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).

Trial Size

530375960

PRS-1210-0.1mg

1 mg/mL

Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.

The immunogen is located within amino acids 240 - 290 of Rubicon.

KIAA0226

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.