alpha-Tubulin Antibody [2B11]

Blue Ice

Predicted: 50 kDa
Observed: 54 kDa

Alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

None

alpha-Tubulin antibody is affinity chromatography purified via peptide column.

1 mg/mL

alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μ g/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin

tubulin, alpha 1a

Optimal dilutions for each application to be determined by the researcher.