RANTES Antibody [5B3-D5]

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of h-RANTES (100ng/mL), a concentration of 5-7 μg/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with our biotinylated antigen affinity purified anti-human RANTES (XP-5257Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Western Blot:

To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

blue ice or room temperature

Chemokines & Cytokines, Functional Assays

6175077

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.