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RANTES Antibody [5B3-D5]

RANTES Antibody [5B3-D5]

Item no.	PRS-XP-5735-M-0.5mg
Manufacturer	ProSci
Amount	0.5 mg
Category	Food Safety Histamines Neuroscience Neuroinflammation Antibodies Western Blot ELISA Primary Antibodies
Type	Antibody Primary
Format	Lyophilized
Applications	WB, ELISA
Clone	5B3-D5
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b
Conjugate/Tag	Unconjugated
NCBI	CCL5
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Regulated upon activation normally T expressed and presumably secreted, CCL5, TCP228, SISd, D17S136E, SCYA5, C-C motif chemokine 5, EoCP, eoCP, RANTES, SIS-delta
Available
Manufacturer - Applications	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of h-RANTES (100ng/mL), a concentration of 5-7 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with our biotinylated antigen affinity purified anti-human RANTES (XP-5257Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Manufacturer - Isotype	RANTES antibody was derived form BALB/c mice and belongs to the IgG2b isotype.
Shipping Temperature	blue ice or room temperature
Storage Conditions	RANTES lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Manufacturer - Research Area	Chemokines & Cytokines, Functional Assays
Background	RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
Protein Gi #	6175077
Immunogen	Monoclonal antibody against human RANTES designated as VL-1 was purified from ascites fluid on a Protein A affinity column.
Clonality	Monoclonal
NCBI Official Name	chemokine (C-C motif) ligand 5
NCBI Organism	Homo sapiens
Disclaimer	This product is for research use only.
User Note	Centrifuge vial prior to opening.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.5 mg

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Delivery expected until 9/4/2025

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