Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb

WB, IHC-P, ICC/IF, IP, ChIP

Histone H2A

Store at -20°C. Avoid freeze/thaw cycles.

Stable for 12 months from date of receipt/reconstitution.

Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Ubiquitination, a post-translational modification in which the ubiquitin is covalently attached to a substrate protein, occurs on histones H2A and H2B. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for gene silencing, X chromosome inactivation, and cellular response to DNA damage. Histone H2A is also monoubiquitinated at Lys119 at sites of DNA damage.

Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb detects histone H2A only when it is ubiquitinated at Lys119.

Ubiquityl

Ubiquitinated human histone H2A (Lys119) peptide

Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Ubiquitination, a post-translational modification in which the ubiquitin is covalently attached to a substrate protein, occurs on histones H2A and H2B. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for gene silencing, X chromosome inactivation, and cellular response to DNA damage. Histone H2A is also monoubiquitinated at Lys119 at sites of DNA damage.