Rabbit anti-Semaphorin 4A Antibody

1mg/mL

Western blot analysis of Semaphorin 4A expression in HeLa (A), COS7 (B) whole cell lysates.

Immunoprecipitation of Semaphorin 4A from 0.5mg Jurkat whole cell extract lysate, using 5ug of anti-Semaphorin 4A Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-Semaphorin 4A Antibody.

Polyclonal

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)