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Rabbit anti-Semaphorin 4A Antibody

Rabbit anti-Semaphorin 4A Antibody

Item no.	YLD7053-100ul
Manufacturer	YL Biotech
Amount	100ul
Category	Antibodies Primary Antibodies
Type	Antibody Polyclonal
Applications	WB, IF, IP, IC
Specific against	Human (Homo sapiens), Monkey (Cynomolgus, Simian)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	SEMAB; SEMB; Semaphorin-4A; Semaphorin-B; Sema B
Available
Background	Rabbit polyclonal antibody to Semaphorin 4A
Concentration	1mg/mL
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Semaphorin 4A. The exact sequence is proprietary.
WB description	Western blot analysis of Semaphorin 4A expression in HeLa (A), COS7 (B) whole cell lysates.
IF/ICC description	Immunofluorescent analysis of Semaphorin 4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IP description	Immunoprecipitation of Semaphorin 4A from 0.5mg Jurkat whole cell extract lysate, using 5ug of anti-Semaphorin 4A Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-Semaphorin 4A Antibody.
Purification_Method	The antibody was purified by immunogen affinity chromatography.
Clonality	Polyclonal
Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution	WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Storage	Store at -20℃. Avoid repeated freeze / thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100ul

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Delivery expected until 12/18/2025

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