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Bovine Cytokine Array C2

Hersteller Raybiotech
Kategorie
Typ Array
Specific against other
Menge 4 Sample Kit
Host Hamster - Armenian
ArtNr AAB-CYT-2-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Number of Targets Detected
10
Species Detected
Bovine (cow)
Compatible Sample Types
Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates
Design Principle
Sandwich-based
Method of Detection
Chemiluminescence
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
Membrane
Gene Symbols
CCL4CXCL10CXCL9IFNA1IFNGIL13IL1AIL21IL36RNTNF
Application Information
Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker/ Key Factor Screening
Identifying Key Factors
Confirming a Biological Process
Kit Components
Bovine Cytokine Antibody Array C2 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-
Conjugated H
R
P
Detection Buffer C
Detection Buffer D
Lysis Buffer8-
Well Incubation Tray
Plastic Sheets
Array Templates
Manual
Other Materials Required
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as U
V
P's Chemi
Doc-
It® or Epi
Chem I
I Benchtop Darkroom or G
E's Image
Quant™ L
A
S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with H
R
P-
Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Product Features
Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 4 Sample Kit
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 28.06.2024 

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