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Human Autophagy Array L1, Glass Slide

Human Autophagy Array L1, Glass Slide

ArtNr	AAH-BLG-AUT-4
Hersteller	Raybiotech
Menge	4 Sample Kit
Quantity options	4 Sample Kit 8 Sample Kit
Kategorie	Arrays Antibody Arrays
Typ	Array
Specific against	Human (Homo sapiens)
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker Screening Identifying Key Factors Confirming a Biological Process Cross-species testing
Manufacturer - Category	Arrays\|Autophagy Arrays\|Antibody Arrays\|Label-Based Antibody Arrays
Manufacturer - Targets	Insulin, AMPKa1, bad, mTOR, PTEN, Bcl-2, ATG10, ATG12, ATG13, ATG3, ATG4A, ATG4B, ATG5, ATG7, GABARAP, LAMP1, RHEB, Cathepsin B, Cathepsin D, Cathepsin L, Ras, BCL2L1, GABARAPL2, HRAS, MAPK1, MAPK8, PIK3CA, RRAGC, AMBRA1, GABARAPL1, ULK2, DAPK3, HIF-1 alpha, HMGB1, IRE1, IRS2, LAMP2, Rab7a, EIF2AK3, EIF2AK4, KIAA1303, PIK3CB, PIK3CD, PIK3R2, PIK3R4, PRKAA2, TBK1, TRAF6, DEPTOR, MAP2K2, NRAS, RRAS2, ATG2A, DAPK2, PIK3C3, RRAGB, CAMKK2, NRBF2, RAB39B, VMP1, WIPI1, TSC1, BNIP3, Rab1A, Akt1, Akt2, GBL, IRS1, PIK3R1, SQSTM1, AKT3, MAP2K1, PPP2CB, PRKACG, RB1CC1, WIPI2, BECN1, EIF2S1, MAPK9, MRAS, PDPK1, PRKACB, RAF1, RPS6KB1, SH3GLB1, SNAP29, STK11, TANK, UVRAG, VAMP8, AKT1S1, ATG14, CFLAR, MAPK3, PIK3R3, PPP2CA, PRKACA, PRKCD, MAP3K7, PRKCQ, IGF-I R, TSC2, PRAP1, STX17
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Description	Label-based antibody array kit using glass slide supports to detect 104 human proteins involved in autophagy. Suitable for serum, plasma, lysates, and cell culture supernatants. Lead Time: 1-2 business days
Number of Targets Detected	104
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
Design Principle	Label-based
Method of Detection	Fluorescence Laser Scanner
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Glass Slide
Gene Symbols	AKT1 \| AKT1S1 \| AKT2 \| AKT3 \| AMBRA1 \| ATG10 \| ATG12 \| ATG13 \| ATG14 \| ATG2A\| ATG3 \| ATG4A \| ATG4B \| ATG5 \| ATG7 \| BAD \| BCL2 \| BCL2L1 \| BECN1 \| BNIP3 \| CAMKK2 \| CFLAR \| CTSB \| CTSD \| CTSL \| DAPK2 \| DAPK3 \| DEPTOR \| EIF2AK3 \| EIF2AK4 \| EIF2S1 \| ERN1 \| GABARAP \| GABARAPL1 \| GABARAPL2 \| HIF1A \| HMGB1 \| HRAS \| IGF1R \| INS \| IRS1 \| IRS2 \| KIAA1303 \| KRAS \| LAMP1 \| LAMP2 \| MAP2K1 \| MAP2K2 \| MAP3K7 \| MAPK1 \| MAPK3 \| MAPK8 \| MAPK9 \| MLST8 \| MRAS \| MTOR \| NRAS \| NRBF2 \| PDPK1 \| PIK3C3 \| PIK3CA \| PIK3CB \| PIK3CD \| PIK3R1 \| PIK3R2 \| PIK3R3 \| PIK3R4 \| PPP2CA \| PPP2CB \| PRAP1 \| PRKAA1 \| PRKAA2 \| PRKACA \| PRKACB \| PRKACG \| PRKCD \| PRKCQ \| PTEN \| RAB1A \| RAB39B \| RAB7A \| RAF1 \| RB1CC1 \| RHEB \| RPS6KB1 \| RRAGB \| RRAGC \| RRAS2 \| SH3GLB1 \| SNAP29 \| SQSTM1 \| STK11 \| STX17 \| TANK \| TBK1 \| TRAF6 \| TSC1 \| TSC2 \| ULK2 \| UVRAG \| VAMP8 \| VMP1 \| WIPI1 \| WIPI2
Kit Components	Ray Bio® L-Series Antibody Array Glass Slide(s) Spin Columns Labeling Reagent Stop Solution Blocking Buffer Wash Buffer 1 Wash Buffer 2 Streptavidin-Conjugated Fluor Adhesive Plastic Strips Labeling Buffer Lysis Buffer30 ml Centrifuge Tube
Other Materials Required	Distilled or de-ionized water Small plastic or glass containers Orbital shaker or oscillating rocker1 ml tube Pipettors, pipette tips and other common lab consumables Aluminum foil Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Purify Samples with Spin Column Biotinylate Samples Purify biotinylated samples using spin column Dry the glass slide Block array surface Incubate with Samples Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
UNSPSC Code	41116133

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