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Human L12 Array, Membrane

Human L12 Array, Membrane

ArtNr	AAH-BLM-12-2
Hersteller	Raybiotech
Menge	2 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit
Kategorie	Arrays Protein Arrays
Typ	Array
Specific against	Human (Homo sapiens)
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker Screening Identifying Key Factors Confirming a Biological Process Cross-species testing
Manufacturer - Category	Arrays\|High-Density Arrays\|Antibody Arrays\|Label-Based Antibody Arrays
Manufacturer - Targets	Bcl-2, SMAD2, BMP-8, Cryptic, Integrin alpha-5, 5T4, ABP1, ACP6, ACYP1, ADA, ADAM-12, AGR3, AIF1L, AK1, AKR1C1, AKR1C4, Alpha-internexin, AmiGO2, Aminopeptidase O, Arginase 1, ASAHL, ASB17, ASF1B, B7-H4, BAI3, BCCIP, BCL2L10, BCS1L, Beta-synuclein, BTN3A1, C1qTNF4, C1qTNF5, CA12, CA5B, CA6, CA8, Cadherin-11, Cadherin-15, Cadherin-17, Cadherin-4, Calnexin, Calreticulin-2, Calsyntenin-2, Carboxylesterase��2, Carboxypeptidase��B1, Caspase-1, Caspase-2, Caspase-9, Caspr2, Cathepsin E, Caveolin-2, Caytaxin, CD164, CD2, CD200 R1, CD205, CD28, CD34, CD4, CD5, CD6, CD7, CD79B, CD99, CD99-L2, CDC14B, CDC25B, CDCP1, CDO, CDX4, CEACAM6, CEACAM7, Cerebellin-2, CES3, CHAT, CHI3L2, CHMP2B, CHST15, CHST2, CHST3, CILP-1, CITED2, Cofilin-2, COL2A1, COMT, Contactin-5, CRELD2, CRISP-2, CRISP-3, CRK, CSNK2A1, CXADR, CXXC1, Cyclin E1, CYFIP2, CYP4F11, CYP4F12, Cytochrome P450 2W1, Cytochrome P450 4B1, Cytohesin-2, Cytohesin-3, DAPK3, DAPP1, DAZL, DCSIGNR, DDR1, DDX21, Desmoglein-3, Desmoglein-4, DNASE1, DNMT3A, DRAK1, Drebrin, DUSP3, Dynactin subunit 2, Dynactin subunit 4, Dynactin subunit 5, DYRK3, Dystrophin, EDEM2, EDIL3, EDNRB, EGR1, EMR2, ENPP5, Enteropeptidase, ENTPD2, Ephrin-A4, Ephrin-A5, Epimorphin (Syntaxin��2), ESR2, Ets-1, FABP6, FABP7, FAM3A, FAM3D, Fc epsilon RI, FES, FGF-3, Fgr, FHL1, Fibromodulin, Ficolin-1, FKBP4, Flotillin-2, FLRT1, FLRT2, FLRT3, FOXC2, FOXJ1, Fucosyltransferase��3, Fucosyltransferase��8, FUT11, FUT7, GAD1, Galectin-4, Galectin-8, Galectin-9, GALNT10, GALNT3, Gastric Intrinsic Factor, GATA-5, GATA1, GATA2, GBA3, GLI-3, GLRX3, Glycophorin-A, Glypican 1, Glypican 2, Glypican-4, GNMT, GPA33, Granzyme B, GRAP2, GRIA2, GRID2, GRK1, GRK5, GRM4, GRM7, HAND1, HAPLN1, HAPLN4, HCN4, Hepsin, Hexokinase-1, HIF-1 alpha, HNF4A, HPGDS, HS3ST1, HS3ST3B1, HS3ST4, HSPH1, ICK, ICOS, IGSF11, IGSF3, IL-28��R alpha (IFN-lambda R1), ING1, Inhibin alpha chain, Integrin alpha-11, Integrin alpha-3, Integrin beta-4, Integrin beta-7, Integrin��alpha 2 (CD49b), Interferon epsilon, IPP2, IRF-2, IRF-4, IRF1, Islet-1, Jagged 1, Jagged 2, JUN, JunB, Kallikrein 1, Kallikrein 12, Kallikrein-15, KPNA2, KRT12, KRT2, Latexin, Leukosialin, LILRA2, LOXL2, LRP chaperone MESD, LRRC4, LRRC8D, LRRTM1, MAP1D, Matrilin-2, Matrilin-3, MDGA1, MDM2, MEF2C, mGluR3, MIA, MKK7, MOG, MyD88, NAGA, NAPSA, NCK1, Nectin-4, NEDD9, Neudesin, Neurofascin, Neurogenin-3, Neuroligin 2, Neuroplastin, NFATC3, NFKB1, Nicastrin, NKp30, NKp44, NLGN4X, NLRP2, NMNAT-1, Norrin, Notch-3, NPDC-1, NQO-1, NTH1, NUDT5, Numb, Olig2, Oligo1, PAR1, Pax-4, Pax3, Paxillin, PILR-alpha, Pirin, PLA2G2A, PLA2G4A, Plexin-A2, PNPLA2, POGLUT1, PPA1, PRKCA, PRKN, Protein Wnt-9a, Protocadherin-1, Protogenin, PSMB8, PTGES2, PTGIS, PTH1R, PTP1B, PTPN11, R-Spondin 3, R-Spondin-2, RAB5A, RalA, RalB, Relaxin-1, Renalase, Renin, RGM-A, RGM-B, RNF113A, RNF31, RNF43, ROBO2, ROBO3, S100 A13, S100 A2, SARS2, SBDS, Secretagogin, Semaphorin 3C, Semaphorin 4C, Semaphorin 4D, Semaphorin 4G, Semaphorin 5A, Semaphorin 6D, Semaphorin-3E, Semaphorin-4A, SERINC3, SH2B1, SH2D1A, Siglec-11, Sirtuin 1, Sirtuin 3, Sirtuin��2, Sirtuin��5, SLC15A1, SLITRK2, SLITRK4, SLITRK5, SMAD3, SMOC-2, SMPD1, SMPD3, SNAP25, SorCS1, SorCS2, SorCS3, Sortilin, SOX10, SOX15, SOX21, SOX9, SPHK1, SPRY1, SRGN, ST3GAL1, ST8SIA1, ST8SIA2, Stabilin-2, Stanniocalcin��1, STUB1, SULT1B1, SULT1C2, SULT2A1, SULT2B1, SULT4A1, SUMF1, SUMF2, SUMO1, SUMO2, Syntaxin 4, Syntaxin��6, TBX18, TC-PTP, TCF7L1, TDP-43, Tenascin R, TFPI-2, THSD1, TINAGL1, TPH1, TPST2, TRAF-3 Isoform 2, Transcobalamin II, Transglutaminase��2 (TGM2), Transglutaminase��4 (TGM4), TREM-2, TrkA, TTK, UCH-L3, UGT1A1, ULBP-3, USE1, VAMP-1, VAMP-2, VAP-B, VDAC2, VIAAT, Wnt-4, WTAP, WWP2, ADAM-23, eNOS / NOS3, ADAM-28, Carbonic Anhydrase XIV / CA14, Carbonic Anhydrase IV / CA4, CD300a / LMIR1, CD39L3 / ENTPD3, CD58 / LFA-3, CD39 / ENTPD1, FOLH1 / PSMA, LRRC32 / GARP, Neuroligin-1 / NLGN1, Siglec-10 / Siglec-G, Siglec-6 / CD327, Testican 1 / SPOCK1, TRP14 / Thioredoxin-like 5, AMSH / STAMBP, Arylsulfatase A / ARSA, ASAH2 / Neutral Ceramidase, CD86 / B7-2, BOC / Brother of CDO, DPPII / QPP / DPP7, Alpha-Galactosidase A / GLA, HIN-1 / SCGB3A1, 15-PGDH / HPGD, JAM-C / CD323, MD-1 / LY86, Meprin beta / MEP1B, RGM-C / Hemojuvelin, FCAR / CD89, SLAM / CD150, Aminopeptidase P2 / XPNPEP2, ARNT / HIF-1 beta, BAMBI / NMA, beta-Glucuronidase / GUSB, C1R / Complement��Component��C1r, CANT1 / SCAN-1, Cathepsin V / Cathepsin L2, CD147 / EMMPRIN, CD27 Ligand / TNFSF7, CD39L2 / ENTPD6, CEACAM-3 / CD66d, CHST1 / GST-1, CHST4 / GST-3, cIAP-1 / BIRC2 / hIAP-2, CIB1 / CIBP, CMG-2 / ANTXR2, C-type Natriuretic Peptide / CNP, CTRC / Chymotrypsin-C / Caldecrin, VCC-1 / CXCL17, DAB2 / DOC-2, Dectin-1 / CLEC7A, DFF45 / ICAD, DLEC / CLEC4C / BDCA-2, Dynactin subunit 1 / DCTN1, DNAM-1 / CD226, DSCAM-L1 / DSCAM2, Endoglycan / PODXL2, ENPP-7 / Alk-Smase, Exostosin-like 3 / EXTL3, FABP8 / M-FABP, FosB / G0S3, FoxM1 / FKHL16, F-Spondin / SPON1, Follistatin-like 4 / FSTL4, FXR / Bile Acid Receptor, Glucokinase / GCK, GLB1 / Beta-galactosidase, Iduronate 2-Sulfatase / IDS, IGSF4C / SynCAM4 / CADM4, IKK-gamma / NEMO / FIP-3, ILT-2 / LIR-1 / CD85j, ILT-4 / LIR-2 / CD85d, Integrin alpha-1 / CD49a, Integrin alpha-4 / CD49d, JAM-B / VE-JAM / CD322, KIRREL3 / NEPH2, L1CAM / NCAM-L1, LILRB4 / CD85k / ILT-3, MD-2 / LY96, MDGA2 / MAMDC1, METTL11A / NRMT, MIS RII / AMHR2, Melan-A / MART-1, MSP Receptor / Ron, Nectin-2 / CD112, NG2 / MCSP, NGL-1 / LRRC4C, NgR3 / NgRH2, NKp46 / NCR1 / CD335, Nogo Receptor / Reticulon-4 Receptor, Neurexin-3 beta / NRXN3, NUP85 / FROUNT, p63 / TP73L / KET, PCK1 / PEPCK-C, PU.1 / Spi-1, RCOR1 / CoREST, Serpin B2 / PAI-2, SIRP-gamma / CD172g, SREC-I / SCARF1, TAZ / WWTR1, Transglutaminase 7 / TGM7, TRACP / PAP / ACP5, ULBP-4 / RAET1E, Fc gamma RI / CD64, PGLYRP4 / PGRP-I beta, VISTA / B7-H5 / PD-1H
Shipping Temperature	Blue ice
Storage Conditions	For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Description	RayBio® L-Series Human Antibody Array 12 Membrane Kit. Detects 500 Human Proteins. Suitable for Serum, Plasma, Lysates, and Cell Culture Supernatants.
Number of Targets Detected	500
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
Design Principle	Label-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	ACP5 \| ACP6 \| ACYP1 \| ADA \| ADAM12 \| ADAM23 \| ADAM28 \| ADGRB3 \| ADGRE2 \| AGR3\| AIF1L \| AK1 \| AKR1C1 \| AKR1C4 \| AMHR2 \| AMIGO2 \| ANTXR2 \| AOC1 \| AOPEP \| ARG1 \| ARNT \| ARSA \| ASAH2 \| ASB17 \| ASF1B \| ATCAY \| BAMBI \| BCCIP \| BCL2 \| BCL2L10 \| BCS1L \| BIRC2 \| BMP8A \| BOC \| BSG \| BTN3A1 \| C1QTNF4 \| C1QTNF5 \| C1R \| CA12 \| CA14 \| CA4 \| CA5B \| CA6 \| CA8 \| CADM4 \| CALR3 \| CANT1 \| CANX \| CASP1 \| CASP2 \| CASP9 \| CAV2 \| CBLIF \| CBLN2 \| CCNE1 \| CD164 \| CD2 \| CD200R1 \| CD226 \| CD28 \| CD300A \| CD34 \| CD4 \| CD5 \| CD58 \| CD6 \| CD7 \| CD70 \| CD79B \| CD86 \| CD99 \| CD99L2 \| CDC14B \| CDC25B \| CDCP1 \| CDH11 \| CDH15 \| CDH17 \| CDH4 \| CDO1 \| CDX4 \| CEACAM3 \| CEACAM6 \| CEACAM7 \| CES2 \| CES3 \| CFC1 \| CFL2 \| CHAT \| CHI3L2 \| CHMP2B \| CHST1 \| CHST15 \| CHST2 \| CHST3 \| CHST4 \| CIB1 \| CILK1 \| CILP \| CITED2 \| CLEC4C \| CLEC4M \| CLEC7A \| CLSTN2 \| CNTN5 \| CNTNAP2 \| COL2A1 \| COMT \| CPB1 \| CRELD2 \| CRISP2 \| CRISP3 \| CRK \| CSNK2A1 \| CSPG4 \| CTRC \| CTSE \| CTSV \| CXADR \| CXCL17 \| CXXC1 \| CYFIP2 \| CYP2W1 \| CYP4B1 \| CYP4F11 \| CYP4F12 \| CYTH2 \| CYTH3 \| DAB2 \| DAPK3 \| DAPP1 \| DAZL \| DBN1 \| DCTN1 \| DCTN2 \| DCTN4 \| DCTN5 \| DDR1 \| DDX21 \| DFFA \| DMD \| DNASE1 \| DNMT3A \| DPP7 \| DSCAML1 \| DSG3 \| DSG4 \| DUSP3 \| DYRK3 \| EDEM2 \| EDIL3 \| EDNRB \| EFNA4 \| EFNA5 \| EGR1 \| ENPP5 \| ENPP7 \| ENTPD1 \| ENTPD2 \| ENTPD3 \| ENTPD6 \| ESR2 \| ETS1 \| EXTL3 \| F2R \| FABP6 \| FABP7 \| FAM3A \| FAM3D \| FCAR \| FCER1A \| FCGR1A \| FCN1 \| FES \| FGF3 \| FGR \| FHL1 \| FKBP4 \| FLOT2 \| FLRT1 \| FLRT2 \| FLRT3 \| FMOD \| FOLH1 \| FOSB \| FOXC2 \| FOXJ1 \| FOXM1 \| FSTL4 \| FUT11 \| FUT3 \| FUT7 \| FUT8 \| GAD1 \| GALNT10 \| GALNT3 \| GATA1 \| GATA2 \| GATA5 \| GBA3 \| GCK \| GLA \| GLB1 \| GLI3 \| GLRX3 \| GNMT \| GPA33 \| GPC1 \| GPC2 \| GPC4 \| GRAP2 \| GRIA2 \| GRID2 \| GRK1 \| GRK5 \| GRM3 \| GRM4 \| GRM7 \| GUSB \| GYPA \| GZMB \| HAND1 \| HAPLN1 \| HAPLN4 \| HCN4 \| HIF1A \| HJV \| HK1 \| HNF4A \| HPGD \| HPGDS \| HPN \| HS3ST1 \| HS3ST3B1 \| HS3ST4 \| HSPH1 \| ICOS \| IDS \| IFNE \| IFNLR1 \| IGSF11 \| IGSF3 \| IKBKG \| INA \| ING1 \| INHA \| IRF1 \| IRF2 \| IRF4 \| ISL1 \| ITGA1 \| ITGA11 \| ITGA2 \| ITGA3 \| ITGA4 \| ITGA5 \| ITGB4 \| ITGB7 \| JAG1 \| JAG2 \| JAM2 \| JAM3 \| JUN \| JUNB \| KIRREL3 \| KLK1 \| KLK12 \| KLK15 \| KPNA2 \| KRT12 \| KRT2 \| L1CAM \| LGALS4 \| LGALS8 \| LGALS9 \| LILRA2 \| LILRB1 \| LILRB2 \| LILRB4 \| LOXL2 \| LRRC32 \| LRRC4 \| LRRC4C \| LRRC8D \| LRRTM1 \| LXN \| LY75 \| LY86 \| LY96 \| MAP2K7 \| MATN2 \| MATN3 \| MDGA1 \| MDGA2 \| MDM2 \| MEF2C \| MEP1B \| MESD \| METAP1D \| MIA \| MLANA \| MOG \| MST1R \| MYD88 \| NAAA \| NAGA \| NAPSA \| NCK1 \| NCR1 \| NCR2 \| NCR3 \| NCSTN \| NDP \| NECTIN2 \| NECTIN4 \| NEDD9 \| NENF \| NEUROG3 \| NFASC \| NFATC3 \| NFKB1 \| NLGN1 \| NLGN2 \| NLGN4X \| NLRP2 \| NMNAT1 \| NOS3 \| NOTCH3 \| NPDC1 \| NPPC \| NPTN \| NQO1 \| NR1H4 \| NRXN3 \| NTHL1 \| NTMT1 \| NTRK1 \| NUDT5 \| NUMB \| NUP85 \| OLIG1 \| OLIG2 \| PAX3 \| PAX4 \| PCDH1 \| PCK1 \| PGLYRP4 \| PILRA \| PIR \| PLA2G2A \| PLA2G4A \| PLXNA2 \| PMP2 \| PNPLA2 \| PODXL2 \| POGLUT1 \| PPA1 \| PPP1R2 \| PRKCA \| PRKN \| PRTG \| PSMB8 \| PTGES2 \| PTGIS \| PTH1R \| PTPN1 \| PTPN11 \| PTPN2 \| PXN \| RAB5A \| RAET1E \| RALA \| RALB \| RCOR1 \| REN \| RGMA \| RGMB \| RLN1 \| RNF113A \| RNF31 \| RNF43 \| RNLS \| ROBO2 \| ROBO3 \| RSPO2 \| RSPO3 \| RTN4R \| RTN4RL1 \| S100A13 \| S100A2 \| SARS2 \| SBDS \| SCARF1 \| SCGB3A1 \| SCGN \| SEMA3C \| SEMA3E \| SEMA4A \| SEMA4C \| SEMA4D \| SEMA4G \| SEMA5A \| SEMA6D \| SERINC3 \| SERPINB2 \| SH2B1 \| SH2D1A \| SIGLEC10 \| SIGLEC11 \| SIGLEC6 \| SIRPG \| SIRT1 \| SIRT2 \| SIRT3 \| SIRT5 \| SLAMF1 \| SLC15A1 \| SLC32A1 \| SLITRK2 \| SLITRK4 \| SLITRK5 \| SMAD2 \| SMAD3 \| SMOC2 \| SMPD1 \| SMPD3 \| SNAP25 \| SNCB \| SORCS1 \| SORCS2 \| SORCS3 \| SORT1 \| SOX10 \| SOX15 \| SOX21 \| SOX9 \| SPHK1 \| SPI1 \| SPN \| SPOCK1 \| SPON1 \| SPRY1 \| SRGN \| ST3GAL1 \| ST8SIA1 \| ST8SIA2 \| STAB2 \| STAMBP \| STC1 \| STK17A \| STUB1 \| STX2 \| STX4 \| STX6 \| SULT1B1 \| SULT1C2 \| SULT2A1 \| SULT2B1 \| SULT4A1 \| SUMF1 \| SUMF2 \| SUMO1 \| SUMO2 \| TARDBP \| TBX18 \| TCF7L1 \| TCN2 \| TFPI2 \| TGM2 \| TGM4 \| TGM7 \| THSD1 \| TINAGL1 \| TMPRSS15 \| TNR \| TP63 \| TPBG \| TPH1 \| TPST2 \| TRAF3 \| TREM2 \| TTK \| TXNDC17 \| UCHL3 \| UGT1A1 \| ULBP3 \| USE1 \| VAMP1 \| VAMP2 \| VAPB \| VDAC2 \| VSIR \| VTCN1 \| WNT4 \| WNT9A \| WTAP \| WWP2 \| WWTR1 \| XPNPEP2
Kit Components	Ray Bio® L-Series Antibody Array Membranes Labeling Reagent Stop Solution Blocking Buffer Detection Buffer C Detection Buffer D Wash Buffer 1 Wash Buffer 2 HRP-Conjugated Streptavidin Concentrate Plastic Sheets Spin Columns Labeling Buffer Lysis Buffer Plastic Incubation Tray
Other Materials Required	Distilled or de-ionized water Small plastic or glass containers Orbital shaker or oscillating rocker Pipettors, pipette tips and other common lab consumables2?5 ml tube15 ml conical collection tube Tissue Paper, blotting paper or chromatography paper A chemiluminescent blot documentation system (such as UVP's Chemi Doc-It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Purify Samples with Spin Column Biotinylate Samples Purify biotinylated samples using spin column Block array membranes Incubate with Samples Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	High density arrays Unbiased detection through direct biotin labeling of samples Low sample consumption High detection sensitivity Same day result Affordable and simple to use
UNSPSC Code	41116133

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