Human Protein Serine/Threonine Phosphorylation Array C1

Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker/ Key Factor Screening
Identifying Key Factors
Confirming a Biological Process

IFN-gamma, TNF alpha, Angiopoietin-1, bFGF, Decorin, IFN-beta, IGF-1, IL-10, IL-13, IL-15, IL-17A, IL-2, IL-21, IL-4, LIF, VEGF-A, EGFR, ErbB3, Erythropoietin, Galectin-3, GM-CSF, HGF, IGFBP-2, IL-12 p40, IL-6, MMP-8, PDGF-BB, RAGE, TGF alpha, IL-5, IL-16, AgRP, Angiopoietin-2, FGF-6, Growth Hormone, IGF-1 R, IGFBP-1, IGFBP-3, IL-11, IL-12 p70, Insulin, Leptin, Leptin R, M-CSF, MIF, Oncostatin M, PDGF-AA, PDGF-AB, Somatotropin, TGF beta 1, TIMP-1, TIMP-2, TNFRSF27, XEDAR, CXCL16, Angiogenin, Angiostatin, EGF, Endostatin, GCSF, GRO alpha/beta/gamma, MMP-1, MMP-9, PLGF, uPAR, VEGF-D, VEGFR2, VEGFR3, bax, IGF-2, IGFBP-4, IGFBP-6, GDNF, TGF beta 2, TGF beta 3, Activin A, Activin B, Activin R2A, Activin R2A/B, ALK-1, Alpha Chain and Full Length, Amphiregulin, Angiopoietin-4, Apelin Receptor, APJ, APRIL, Artemin, AXL, BACE-1, BD-1, BDNF, beta-Catenin, beta-NGF, BIK, BLR-1, BMP-2, BMP-3, BMP-3b, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMPR-II, CCN2, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD163, CD91 Cluster II, Cerberus 1, CF10, Chem R23, CLC, CNTF, CNTF R alpha, CRAM-A/B, CRIM1, Cripto-1, CRTH-2, Cryptic, CSFR1, CSK, CTGF, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, D6, DAN, DANCE, DKK-1, Dkk-3, Dkk-4, Dtk, E-Selectin, EDA-A2, EDAR, EDG-1, EMAP-II, EML2, Endocan, Endothelin-1, Epiregulin, ErbB1, ErbB2, FACX, FADD, FAM3B, FGF R3, FGF R4, FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-8, FGF-9, FGF-BP, FIL1 delta, FIL1 epsilon, FIL1 eta, FIL1 zeta, FLRG, Flt-3 Ligand, Follistatin, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Fusin, GDF-1, GDF-10, GDF-15, GDF-3, GDF-9, GFR alpha-2, GFR alpha-4, Glucagon, Glut1, Glut2, Glut3, Glut5, Glyoxalase 1, Glypican 3, Glypican 5, GM-CSF R alpha, Granzyme A, Grb2, Gremlin-1, Growth Hormone R, HB-EGF, HCR, ICAM-5, IDE, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-gamma R1, IGF-2 R, IL-17B, IL-17C, IL-17D, IL-17F, IL-19, IL-20, IL-22, IL-22BP, IL-23, IL-24, IL-27, IL-3, IL-31, IL-7, IL-9, Insulysin, ITGAL, Kremen-1, Kremen-2, LBP, LCK, LECT2, Lefty-A, LFA-1 alpha, LIF R alpha, Lipocalin-1, LRP-1 Cluster II, LRP-6, LTBP1, Lymphotoxin beta, M-CSF R, MADH9, MFG-E8, MFRP, MICA, MIP-2, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, MMP-15, MMP-16, MMP-19, MMP-2, MMP-20, MMP-24, MMP-25, MMP-3, MMP-7, MSP alpha, MT3-MMP, MT5-MMP, MT6-MMP, MuSK, Neuritin, NeuroD1, Neuropilin-2, Neurturin, Nidogen-1, Nr-CAM, NRG1 Isoform GGF2, NRG2, NRG3, NT-3, NT-4, Orexin-A, Orexin-B, Osteocrin, P-Selectin, PDGF R alpha, PDGF R beta, PDGF-C, PDGF-D, Peroxiredoxin-6, Persephin, PLUNC, Prdx6, Progranulin, Prolactin, RELM beta, ROBO4, S100 A10, S100 A8/A9, sFRP-1, sFRP-3, sFRP-4, SIGIRR, SLPI, SMAD1, SMAD4, SMAD5, SMAD7, SMAD8, SMAD9, Spinesin, TCCR, TFPI, TGF beta R2, TGF beta R3, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymopoietin isform alpha, Thyroid Peroxidase, Tie-1, TIMP-3, TIMP-4, TL1A, TLR1, TLR2, TLR3, TLR4, TMEFF2, TNFRSF12A, TNFSF15, TNFSF3, TP, TPX, TRADD, TREM-1, TSG-6, TSLP R, TYRO3, Ubiquitin+1, uPA, Vasorin, VEGF-B, VEGF-C, WIF-1, WSX-1, IL-17RA, IL-17RB, IL-17RD, IL-23R, IL-4R, Eotaxin-1 / CCL11, Fas Ligand / TNFSF6, IL-8 / CXCL8, MIP-1 alpha / CCL3, RANTES / CCL5, Activin R1A / ALK-2, CTLA-4 / CD152, BLC / CXCL13, NCAM-1 / CD56, TNF R1 / TNFRSF1A, IL-1 beta / IL-1F2, CD40 Ligand / TNFSF5, MIG / CXCL9, MIP-1 beta / CCL4, Angiopoietin-like protein 2, Angiopoietin-like protein 7, CD27 / TNFRSF7, VE-Cadherin / CDH5, Chordin-like 2 / CHRDL2, CXCL2 / CINC-3 / MIP2, Coagulation Factor X / F10, Follistatin-like 1 / FSTL1, GFR alpha-3 / GDNF R alpha-3, IL-10R alpha, IL-10R beta, IL-12R beta 1, IL-12R beta 2, IL-13R alpha 2, IL-15R alpha, IL-17RC, IL-18BPa, IL-38 / IL-1F10, IL-1R-1 / IL-1RT-1 / IL-1R alpha / CD121a / p80, IL-1R-2 / IL-1RT-2 / IL-1R beta / CD121b, IL-20R alpha, IL-20R beta, IL-21R, IL-22R alpha 1, IL-27R alpha, IL-2R alpha, IL-31R alpha, IL-36 beta / IL-1F8, IL-3R alpha, IL-5R alpha, IL-6R, Insulin R / CD220, RELT / TNFRSF19L, Siglec-9 / CD329, SPARC / Osteonectin, TGF beta R1 / ALK 5, Tie-2 / TEK, BAFF R / TNFRSF13C, TRANCE / TNFSF11, SCF / KITLG, Angiopoietin-3 / ANGPTL1, Activin C / INHBC, BMPR-IA / ALK-3, PECAM-1 / CD31, BRAK / CXCL14, FGF-10 / KGF-2, GDF-11 / BMP-11, GDF-8 / Myostatin, GFR alpha-1 / GDNF R, ICAM-2 / CD102, IL-13R alpha 1, IL-1R-4 / ST2 / IL-33R, IL-36 gamma / IL-1F9, LIGHT / TNFSF14, MCP-3 / MARC / CCL7, SAA / Serum Amyloid A, Siglec-5 / CD170 / Siglec-F, TMEFF1 / Tomoregulin-1, TNF R2 / TNFRSF1B, WISP-1 / CCN4, IL-1 alpha / IL-1F1, IP-10 / CXCL10, TWEAK / TNFSF12, ALCAM / CD166, CD40 / TNFRSF5, Endoglin / CD105, ErbB4 / HER4, GITR / TNFRSF18, IL-7R alpha / CD127, Lymphotoxin beta R / TNF RIII / TNFRSF3, GASP-1 / WFIKKNRP, IL-36Ra / IL-1F5, IL-1ra / IL-1RN / IL-1 F3 / IRAP, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, FGF-7 / KGF, HGFR / MET, TWEAK R / TNFRSF12, Fas / TNFRSF6 / Apo-1, MIP-3 alpha / CCL20, Pentraxin-3 / TSG-14, 6Ckine / CCL21, Activin R1B / ALK-4, Adiponectin / ACRP30, ALK-6 / BMPR-IB, BMP-15 / GDF-9B, Betacellulin / BTC, MEC / CCL28, CD30 / TNFRSF8, CD30 Ligand / TNFSF8, CD80 / B7-1, Chordin-Like-1 / CHRDL1, Coagulation Factor III / Tissue Factor / Thromboplastin / F3, Cardiotrophin-1 / CT-1, CTACK / CCL27, Crossveinless-2 / CV-2 / BMPER, DcR3 / TNFRSF6B, DR3 / TNFRSF25, DR6 / TNFRSF21, EG-VEGF / PK1, ENA-78 / CXCL5, EN-RAGE / S100 A12, Eotaxin-2 / MPIF-2 / CCL24, Eotaxin-3 / CCL26, FGF R5 / FGFRL1, Fractalkine / CX3CL1, GASP-2 / WFIKKN, GCP-2 / CXCL6, GCSF R / CD114, GITR Ligand / TNFSF18, HCC-1 / CCL14, HCC-4 / CCL16, Hepassocin / HFREP-1, HVEM / TNFRSF14 / CD270, I-309 / TCA-3 / CCL1, ICAM-1 / CD54, ICAM-3 / CD50, IGFBP-rp1 / IGFBP-7, IL-17E / IL-25, IL-18R beta / IL-1R-7 / AcPL / IL-18RAcP, IL-36 alpha / IL-1F6, IL-37 / IL-1F7, IL-36 gamma / IL-1RP2 / IL-1F9, IL-1R-3 / IL-1RAcP, IL-18 R alpha / IL-1R-5, IL-1Rrp2 / IL-1R-6, IL-26 / AK155, IL-28A / IFN lambda 2, IL-29 / IFN-lambda 1, IL-2R beta / CD122, I-TAC / CXCL11, Integrin alpha-M / CD11b / MAC-1, Kininogen / Kininostatin, Lipocalin-2 / NGAL, L-Selectin / CD62L, Lymphotactin / XCL1, MCP-4 / CCL13, MDC / CCL22, MIP-1 delta / CCL15, MIP-3 beta / CCL19, NAP-2 / PPBP / CXCL7, NGFR / TNFRSF16, NOV / CCN3, NRG1-alpha / HRG1-alpha, NRG1-beta 1 / HRG1-beta 1, Osteoprotegerin / TNFRSF11B, Osteoactivin / GPNMB, OX40 Ligand / TNFSF4, PARC / CCL18, Platelet Factor 4 / CXCL4, Pref-1 / DLK-1 / FA1, RANK / TNFRSF11A, SCF R / CD117 / c-kit, gp130 / sgp130, Soggy-1 / DkkL1, Sonic Hedgehog N-Terminal / Shh-N, Siglec-5 / CD170, TACI / TNFRSF13B, TARC / CCL17, TECK / CCL25, TNF beta / TNFSF1 / LT-alpha, BCMA / TNFRSF17, Thrombopoietin / TPO, TRAIL / TNFSF10, TRAIL R1 / TNFRSF10A / DR4, TRAIL R2 / TNFRSF10B / DR5, TRAIL R3 / TNFRSF10C, TRAIL R4 / TNFRSF10D, TROY / TNFRSF19, PD-ECGF / Thymidine Phosphorylase, VCAM-1 / CD106, TCK-1 / CXCL7, IL-2R gamma / Common gamma Chain, IL-22R alpha 2, GDF-5 / BMP-14, IL-1F10 / IL-1HY2 / FIL1 theta, IL-1-RAPL-1, IL-1R-8, IL-1R-9 / IL-1-RAPL-2, IL-8R A / CXC-R1, IL-8R B / CXC-R2, SDF-1 / CXCL12, TGF beta 5, Thymopoietin / LAP2

-20°C

RayBio® C-Series Human Protein Serine/Threonine Phosphorylation Array 1 Kit. Detects 507 Serine/Threonine-phosphorylated proteins in human samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates

Chemiluminescence

Membrane

Human Protein Serine/Threonine Phosphorylation Array C1 Membranes
Blocking Buffer
Biotinylated Anti-Phosphotyrosine Antibody1, 000
X HRP-Streptavidin Concentrate20
X Wash Buffer I Concentrate20
X Wash Buffer I
I Concentrate2
X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
Incubation Tray w/ Lid
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail IIPlastic Sheets
Array Map Template
User Manual

Blocking
Sample Incubation
First Wash
Biotinylated Antibody Cocktail Incubation
Second Wash
HRP-Streptavidin Incubation
Third Wash
Chemiluminescence Detection

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