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Rat Tyrosine Phosphorylation Array G2

Rat Tyrosine Phosphorylation Array G2

ArtNr	AAR-PTYR-G2-4
Hersteller	Raybiotech
Menge	4 Sample Kit
Quantity options	4 Sample Kit 8 Sample Kit
Kategorie	Arrays Antibody Arrays
Typ	Array
Specific against	Rat (Rattus norvegicus)
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker Screening Identifying Key Factors Confirming a Biological Process
Manufacturer - Category	Arrays\|Post-Translational Modification Arrays\|Phosphorylation Arrays\|Antibody Arrays\|Glass Slide Antibody Arrays
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Number of Targets Detected	500
Compatible Sample Types	Cell Lysates Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Fluorescence Laser Scanner
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Glass Slide
Gene Symbols	Acaca \| Acp1 \| Acta2 \| Actc1 \| Actn1 \| Acvr1c \| Acvr2a \| Adam10 \| Adamts1 \| Adgrl3\| Adipoq \| Agrn \| Ak1 \| Alcam \| Andpro \| Angpt1 \| Anpep \| Anxa1 \| Anxa5 \| Apex1 \| Ar \| Arg1 \| Asah2 \| B2m \| Bad \| Bcam \| Bcl10 \| Bcl2l2 \| Bglap \| Bid \| Bmp2 \| Bst1 \| Btla \| C1qbp \| Cadm3 \| Cat \| Cav2 \| Ccdc22 \| Cckar \| Ccl11 \| Ccl27 \| Ccl3 \| Ccl5 \| Ccl7 \| Cd14 \| Cd200 \| Cd27 \| Cd276 \| Cd300lf \| Cd38 \| Cd48 \| Cd5 \| Cd63 \| Cd86 \| Cd8a \| Cd93 \| Cdc25b \| Cdc37 \| Cdh1 \| Cdh17 \| Cdh2 \| Cdh3 \| Cdh4 \| Cdkn1b \| Cdnf \| Ces1c \| Chrd \| Clu \| Cntf \| Cntn1 \| Cntn2 \| Cntn4 \| Cntnap5b \| Colec12 \| Cpa1 \| Cpa2 \| Cpb1 \| Creld2 \| Crkl \| Cryab \| Cst3 \| Ctgf \| Cthrc1 \| Ctsb \| Ctsc \| Ctse \| Ctsz \| Cubn \| Cxcl1 \| Cxcl10 \| Cxcl16 \| Cxcl9 \| Cycs \| Dcn \| Dhfr \| Dlk1 \| Dll1 \| Dnajb11 \| Dyrk3 \| Ecm1 \| Efna1 \| Efnb1 \| Egf \| Egfr \| Eif5a \| Eno1 \| Entpd2 \| Epha5 \| Ephb1 \| Ephb6 \| Epo \| Erbb2 \| Erbb3 \| Errfi1 \| Esr1 \| Ets1 \| F11r \| F2 \| Fabp2 \| Fabp3 \| Fabp5 \| Fetub \| Fgf1 \| Fgf12 \| Fgf2 \| Fgfr4 \| Fgr \| Fkbp1a \| Fkbp1b \| Fkbp4 \| Flt1 \| Fmod \| Frk \| Gabra4 \| Gad1 \| Gapdh \| Gas1 \| Gdf11 \| Gdnf \| Gfap \| Gfra1 \| Ggt1 \| Gh1 \| Git1 \| Glg1 \| Glo1 \| Glrx3 \| Got2 \| Gpc3 \| Gpt \| Gpx1 \| Gpx3 \| Grb2 \| Grin2a \| Grk1 \| Grk2 \| Grk5 \| Gzmb \| Haao \| Hagh \| Havcr1 \| Hgf \| Hif1a \| Hmox2 \| Hpx \| Hrg \| Hsd11b1 \| Hsp90ab1 \| Hspa1a \| Hspa9 \| Hspb1 \| Hspb6 \| Hspd1 \| Hsph1 \| Htra2 \| Ifng \| Igf1 \| Igfbp5 \| Il10 \| Il11ra1 \| Il12a \| Il13ra2 \| Il18 \| Il1b \| Il1r1 \| Il1r2 \| Il1rn \| Il2 \| Il21 \| Il22 \| Il23a \| Il2ra \| Il4 \| Il4r \| Il6 \| Il6st \| Il7 \| Il9 \| Ilk \| Ilkap \| Impdh1 \| Inppl1 \| Isl1 \| Jag1 \| Jam3 \| Kdr \| Keap1 \| Klkb1 \| Kng1 \| Lair1 \| Lamtor3 \| Lcn2 \| Lenep \| Lepr \| Lgals1 \| Lgals3 \| Lgals4 \| Lgmn \| Lif \| Lifr \| Lrp4 \| Lyn \| Lypd3 \| Maea \| Mag \| Maged1 \| Map2k2 \| Mapk12 \| Mapk9 \| Mbl2 \| Mcam \| Mif \| Mmp2 \| Mmp8 \| Mmp9 \| Mog \| Mospd1 \| Ncam1 \| Ncan \| Ncr3 \| Nedd8 \| Nes \| Nfasc \| Nfe2l2 \| Nfkbib \| Nlgn1 \| Nlgn2 \| Nope \| Nos1 \| Notch1 \| Notch2 \| Notch3 \| Nptn \| Nptx2 \| Nrgn \| Nrn1 \| Nrp1 \| Nrxn1 \| Ntn1 \| Ntrk1 \| Ntrk3 \| Nup85 \| Nxph1 \| Olfm1 \| Olr1 \| Pak1 \| Pak5 \| Pck1 \| Pdgfb \| Pdgfra \| Pecam1 \| Pfkm \| Pgc \| Pigr \| Plaur \| Pon3 \| Por \| Ppbp \| Ppia \| Ppib \| Ppm1a \| Ppp1r2 \| Ppp3r1 \| Prdx1 \| Prdx2 \| Prdx4 \| Prdx6 \| Prkaa2 \| Prkaca \| Prkca \| Prkce \| Prkci \| Prl \| Prl8a4 \| Procr \| Psap \| Psma1 \| Psma2 \| Ptk2 \| Ptpn1 \| Ptpn2 \| Ptpn6 \| Ptprf \| Rala \| Rbp4 \| Reg3a \| Reg3b \| Ren1 \| Rhd \| Robo1 \| Rock2 \| Rtn4 \| Scgn \| Sdc1 \| Sdc2 \| Sema4c \| Sema4d \| Sema5a \| Sema7a \| Serpina1 \| Serpine1 \| Serpinf1 \| Serpinf2 \| Sftpd \| Sh2b1 \| Slit3 \| Smad3 \| Smad7 \| Smarcd2 \| Smc1a \| Snca \| Sort1 \| Sost \| Sox10 \| Src \| Stat3 \| Stx1a \| Tafa5 \| Tagln \| Tek \| Tf \| Tgfbr3 \| Tnf \| Tnfrsf12a \| Tnfrsf1a \| Tnfrsf4 \| Tnfrsf9 \| Tollip \| Tp53 \| Tpt1 \| Trem1 \| Trhde \| Txn2 \| Uchl1 \| Uchl3 \| Unc5a \| Unc5b \| Vamp2 \| Vcl \| Vsig1 \| Wfdc2 \| Wnt5a \| Xiap \| Ywhae \| Ywhah \| Ywhaq
Kit Components	Rat Tyrosine Phosphorylation Antibody Array G2 Slide(s)2 X Cell Lysis Buffer Protease Inhibitor Cocktail Phosphatase Inhibitor Cocktail Set IIBlocking Buffer20 X Wash Buffer I20 X Wash Buffer IIBiotin-Conjugated Anti-phosphotyrosine Fluorescent dye-Conjugated Streptavidin Wash Buffer III Adhesive film
Other Materials Required	Shaker Laser scanner for fluorescence detection Aluminum foil Distilled water Plastic box50 ml Centrifuge tube Isopropanol (2-propanol)
Protocol Outline	Dry the Glass Slide Blocking and Incubation Fluorescence Detection
Product Features	High sensitivity and specificity Low sample volume (10-100 µl per array) Large dynamic range of detection Compatible with most sample types Test 4 or 8 samples on each slide Suitable for high-throughput assays
UNSPSC Code	41116133
Introduction	RayBiotech is committed to develop a series of phosphorylation antibody arrays. RayBio® Rat Protein Tyrosine Phosphorylation Antibody Array 2 is specifically designed for simultaneous identification of the relative levels of phosphorylation of 500 different rat proteins in cell lysate. By monitoring the changes in protein tyrosine phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot. To use the Rat Protein Tyrosine Phosphorylation Antibody Array 2, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and biotinylated anti-phosphotyrosine antibodies are then used to detect the phosphorylated tyrosines on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.

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