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RayPlex Human Mesenchymal Stem Cell Bead Array 1

RayPlex Human Mesenchymal Stem Cell Bead Array 1

ArtNr	FAH-MSC-1-480
Hersteller	Raybiotech
Menge	480 tests
Quantity options	192 tests 48 tests 480 tests 96 tests
Kategorie	Arrays Antibody Arrays
Typ	Array
Specific against	Human (Homo sapiens)
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Multiplexed Protein Detection Biomarker Screening Identifying Key Factors Confirming Biological Process Biomarker Validation Validation of Antibody Array Results Quantitative Protein Detection Establishing Normal Range
Manufacturer - Category	Arrays\|Cytometric Bead Arrays\|Antibody Arrays\|Stem Cell Arrays
Manufacturer - Targets	VEGF-A, IL-6, TGF beta 1, Eotaxin-1 / CCL11, IL-8 / CXCL8, MIP-1 alpha / CCL3, RANTES / CCL5, MIP-1 beta / CCL4, IP-10 / CXCL10, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, ICAM-1 / CD54
Shipping Temperature	Blue ice
Storage Conditions	4°C
Research Area	Stem Cells
Description	This RayPlex® kit is a cytometric bead array to quantitatively measure 13 human biomarkers associated with mesenchymal stem cells by flow cytometry. The assay has been optimized for serum, plasma, and cell culture supernatant.
Number of Targets Detected	13
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Design Principle	Sandwich-based
Method of Detection	Flow Cytometry
Quantitative/Semi-Quantitative	Quantitative
Solid Support	Fluorescent Microbeads
Gene Symbols	CCL11 \| CCL2 \| CCL3 \| CCL4 \| CCL5 \| CXCL1 \| CXCL8 \| ICAM1 \| IL10 \| IL6\| TGFB1 \| VEGFA
Kit Components	RayPlex„¢ Multiplex Bead Cocktail1 X Assay Diluent20 X Wash Buffer Ray Bio® Serum Diluent Lyophilized Protein Standard Mix1 X Biotinylated Detection Antibody Cocktail10 X Streptavidin-PEFlow Cytometer Setup Bead Cocktail V-bottom 96-well Microplate Filter 96-well Microplate
Other Materials Required	Orbital 96-well plate shaker (with ability to reach 1000 rpm) Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (AP C) channels Aluminum foil or 96-well Plate Aluminum Sealers Distilled water 1.5 ml polypropylene microcentrifuge tubes or similar Microcentrifuge If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate Optional: 96-well round bottom plate (to prepare samples) Optional: High throughput sampler (HT S) for 96-well plate reading Don't have a compatible flow cytometer? Ray Biotech provides full testing services for RayPlex„¢ bead arrays. Get a quote!
Protocol Outline	Prepare Reagents, Standards, and Samples Add RayPlex„¢ Multiplex Bead Cocktail to each well Incubate with Samples and Standards Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluorophore Analyze with a compatible flow cytometer Perform data analysis
UNSPSC Code	41116133

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.