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Human Glycosylation Array L3

Human Glycosylation Array L3

ArtNr	GAH-GCM-L3-8
Hersteller	Raybiotech
Menge	8 Sample Kit
Quantity options	4 Sample Kit 8 Sample Kit
Kategorie	Arrays Protein Arrays
Typ	Array
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Identify and profile the glycans in their samples Determine whether their biomarker of interest has glycan moieties Determine relative expression levels
Manufacturer - Category	Arrays\|Glycobiology Arrays
Manufacturer - Targets	Growth Hormone, bad, Bcl-w, BID, Cytochrome C, alpha-Synuclein, DAN, FSH, GRP, 14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, 53BP1, ABAT, ABCF1, ABI3BP, ACAA, ACAA1, ACAA2, ACACA, ACLP, ACLY, Aconitase 1, ACTBL2, ACTC1, Actinin alpha 1, ADAS, ADH1B, ADH1C, ADH4, ADH5, ADM, Advillin-N-t, AFG3L2, AGA, Aggrecan, AGXT, AHNAK, Ahsp, AIF, AK2, AKAP9, AKR1B1, AKR1C3, AKR7A2, ALAD, Alanine Transaminase, Alcohol Dehydrogenase, Aldehyde Oxidase 1, ALDH16A1, ALDH1A1, ALDH9A1, ALP, alpha 1, 2 Mannosidase IA, alpha Actinin 4, Alpha Fodrin, alpha Glucosidase II, Alpha Tubulin, AlphaA Crystallin, ALS, ALS+B62:B1212CR1, Als2, Aminopeptidase��PILS, Androgen Receptor, ANK, Ankrd26, Annexin A1, Annexin A2, Annexin A6, ANP, APA, APM2, ARFBP1, ARFGEF3, Argininosuccinate Lyase, ArgRS, ARP19, Arp2, ARP2/3, Arp3, ARPC2, ARPC3, ART3, ARX, ASH2L, Asialoglycoprotein Receptor 2, ASK1, Aspartate Aminotransferase, Aspartyl Aminopeptidase, ASXL1, ATBF1, ATP5A, ATP5O, ATPB, B3GNT2, B4GalT1, Band 3, BASP1, Bassoon, BAZ2B, BCHE, BCOR, beta 1 Spectrin, beta B1 Crystallin, BIN2, BIRC6, BLMH, BLVRB, BPGM, BPIFB1, BPIL1, BRCA 2, BRD2, Brevican, Brg1, BRSK1, BTD, BTF3, C1Q, C1QA, C1QB, C1QR1, C1RL, C3orf75, C4BPA, C5b-9, C6 -N-terminal, C8G, C9orf40, CA1, CA150, CA2, CA3, CACNB4, CAD, Cadherin-22, Caldesmon, CALML5, Calmodulin, Calpain 1, Calpain S1, Calpastatin, Calretinin, Calumenin, CAP1, CapG, CAPZA1, Carboxylesterase��1, Carboxypeptidase��E, CARHSP1, Caspase-14, Catalase, Catenin gamma, Cathelicidin, Cathepsin A, Cathepsin G, Cathepsin H, CBS, CCDC126, CCDC25, CCT3, CD109, CD133, CD16, CD32, CD35, CD41, CD5L, CD9, CD98, CDA, CDC5L, CDK2, CEACAM-8, CECR1, CENPF, CEP57, CETP, Cezanne, CFHR 1, CFHR4, CFI, CFL1, CHC17, Chitobiase, Chitotriosidase, CHORDC1, CHREBP, Chromogranin B, Chromogranin C, CIP29, CKB, CLIC1, CLIC4, CLIP170-N-t, CLPS, CLTA, CNN2, CNOT1, CO4A2, Coagulation Factor IX, Coagulation Factor V, Coagulation Factor XII, Coagulation Factor XIII, COG4, COL19A1, COL4A3, COL6A2, COL9A3, COLEC10, Collagen I alpha-1, Collagen III, Collagen IV alpha-6, Collagen IX, Collagen V, Collagen VI, Collagen X, Collagen XV alpha 1, Complement Factor B, COPS8, Corneodesmosin, Coronin 3, Cortactin, COTL1, CPEB3, CPN1, CPNE3, CPS1, Creatine Kinase MM, CRF21, CRHBP, CrkL, CRMP2, CRTAC1, CS, Ctip2, Cux2, Cyclophilin B, Cyclophilin��A, Cystatin D, Cystatin E/M, Cystatin SN, Cystatin��S, Cysteine-rich Protein 1, CYTL1, Cytochrome b5, Cytokeratin 1, Cytokeratin 10, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 9, D4-GDI, DAK, DARS2, DCI, DCXR, DDAH1, DDT, DDX3Y, DEFA6, Der p2, Dermcidin, Desmocollin-1, Desmocollin-2, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoplakin, Desmuslin, Destrin, DGK, DISC 1, DMGDH, DMRN9, Dopamine beta Hydroxylase, DOT1L, DPEP2, DPP3, DPPI, DRIL1, DSCAM, DSPG3, Dystroglycan, ECHS1, ECM-1, EEF1G, EEF2, EFEMP2, EFTUD2, EHD1, EHD3, EIF3S2, eIF4A1-N-terminal, eIF5A, ELAVL1, EMILIN1, EMSY, EN2, ENO1, ENO1/2/3, ENSA, Envoplakin, Eosinophil derived neurotoxin, EPB41, EPCR, Ephrin-B1, Ephrin-B2, EPHX2, EPPK1, Eps 15, ERAB, ERp29, ERp57, ERp72, ESD, ESR1, ETL, EVC2, Ezrin, F-box protein 38, FAM20C, FAM3C, Fascin, FASN, fast skeletal Myosin, FASTKD5, FBPase 1, FBPase 2, FCGBP, FDPS, FH, Fibrillin 1, Fibrinogen gamma chain, Fibrinogen-like 2, Fibrinopeptide B, Fibulin 3, Ficolin-2, Filamin A, Filamin B, Filamin C, FKBP12, FKBP25, FKBP51, FLG2, FOLR3, Frizzled 8, FRY, FTL, FUCA1, FUCA2, Fumarylacetoacetate hydrolase, G0/G1switch 2, G3BP, G6PD, GALNT2, GAPHD, GARNL1, GART, Gastrokine 1, GATM C-terminal, GBE1, GCDFP15, GCLC, GCSH, GDA, GDI1, GDI2, Gephyrin, GFAP, GHRF, GIP, GLIPR2, GLRX1, Glucosidase 2 subunit beta, GLUD1, Glutamyl hydrolase gamma, Glutathione Synthetase, Glycerol 3 Phosphate Dehydrogenase, Glyoxalase II, GM2A, GMF beta, GNB1, GNPTG, GOLPH4, GOT2, GPCR GPR116, GPLD1, Grainyhead-like protein 1 homolog, Granzyme M, GRHPR, GSTM1, GSTO1, GSTP1, Guanylin, GULP1, H6PD, HABP2, HBZ, HCFC1, HDGF, HEG1, Hemoglobin, Hemoglobin A1c, Hemoglobin subunit beta, Hemoglobin subunit delta, Hemoglobin subunit gamma 2, HEXB, HGFA, hHR23b, HIBADH, HINT1, HIP1R, Histone H1.2, Histone H1.3, Histone H2A, Histone H2A.Z, Histone H2B K, Histone H3.3, Histone H4, HLA-C, HMGB1, HMGB2, HMGB3, HMGN2, HN1, HNF-3 alpha, hnRNP A1, hnRNP A2B1, hnRNP C1/C2, hnRNP G, hnRNP L, hnRNP M1-M4, hnRNP U, Hornerin, HOXB3, HOXD11, HP1BP3, HPD, HPR, HPRG, HPRT, HRSP12, HSC70, HSP47, LAMR1, Tubulin beta-1 chain, Tubulin beta-3 chain, Ubiquitin-activating enzyme E1, ADP-Ribosyl Cyclase 2 / BST-1 / CD157, Cathepsin Z / Cathepsin X / Cathepsin P, Coagulation Factor VII / F7, Coagulation Factor XI / F11 / PTA, Carboxypeptidase M / CPM, DEP-1 / CD148, Human Amyloid-like protein 1 / APLP-1, Apolipoprotein(a), B7-H3 / CD276, Cadherin-6 / KCAD, Collectin-12 / CL-P1, Contactin-3 / BIG-1, FABP5 / E-FABP, CD155 / PVR, Aminoacylase-1 / ACY1, Alkaline Phosphatase / ALPL, Annexin A5 / Annexin V, Aminopeptidase LRAP / ERAP2, Antithrombin 3 / Serpin C1, B7-H2 / ICOS Ligand, C1S / Complement��Component��C1s, C4.4A / LYPD3, Carboxypeptidase B2 / CPB2, CD21 / CR2 / EBV receptor, CD39L4 / ENTPD5, CD42b / GPIb-alpha, CD48 / SLAMF2, Complement Factor H-related 5 / CFHR5, Cytokeratin 20 / KRT20, Endorepellin / Perlecan, GOLM1 / GP73, GPV / CD42d, Thrombospondin 5 / COMP, ADAM DEC1, Angiopoietin-like protein 6, Angiopoietin-like protein 8, BMP-1 / PCP, GDF-7 / BMP-12, Apolipoprotein A-I-binding protein / APOA1BP, Apolipoprotein F / ApoF, Apolipoprotein L1 / ApoL1, Apolipoprotein L2 / ApoL2
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Research Area	Post-Translational Modifications, Glycosylation
Description	RayBio® Human Glycosylation Antibody Array L3. For the simultaneous detection of the glycosylation protein profile of 500 human proteins in serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.
Number of Targets Detected	500
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Fluorescence Laser Scanner
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Glass Slide
Gene Symbols	ABAT \| ABCF1 \| ABI3BP \| ACAA1 \| ACAA2 \| ACACA \| ACAN \| ACAT2 \| ACLY \| ACO1\| ACTBL2 \| ACTC1 \| ACTN1 \| ACTN4 \| ACTR2 \| ACTR3 \| ACY1 \| ADAMDEC1 \| ADGRF5 \| ADGRL4 \| ADH1A \| ADH1B \| ADH1C \| ADH4 \| ADH5 \| ADIRF \| ADM \| AEBP1 \| AFG3L2 \| AGA \| AGPS \| AGXT \| AHNAK \| AHSP \| AIFM1 \| AK2 \| AKAP9 \| AKR1B1 \| AKR1C3 \| AKR7A2 \| ALAD \| ALDH16A1 \| ALDH1A1 \| ALDH9A1 \| ALPL \| ALS2 \| ANGPTL6 \| ANGPTL8 \| ANK1 \| ANKRD26 \| ANXA1 \| ANXA2 \| ANXA5 \| ANXA6 \| AOX1 \| APLP1 \| APOF \| APOL1 \| APOL2 \| AR \| ARFGEF3 \| ARHGDIB \| ARID3A \| ARPC1B \| ARPC2 \| ARPC3 \| ARPP19 \| ART3 \| ARX \| ASGR2 \| ASH2L \| ASL \| ASXL1 \| ATP5A1 \| ATP5B \| ATP5O \| AVIL \| B3GNT2 \| B4GALT1 \| BAD \| BASP1 \| BAZ2B \| BCAN \| BCHE \| BCL11B \| BCL2L2 \| BCOR \| BID \| BIN2 \| BIRC6 \| BLMH \| BLVRB \| BMP1 \| BPGM \| BPIFB1 \| BPIFB2 \| BRCA2 \| BRD2 \| BRSK1 \| BSN \| BST1 \| BTD \| BTF3 \| C1QA \| C1QB \| C1QC \| C1RL \| C1S \| C4BPA \| C6 \| C8A \| C8G \| C9ORF40 \| CA1 \| CA2 \| CA3 \| CACNB4 \| CAD \| CALB2 \| CALD1 \| CALM1 \| CALML5 \| CALU \| CAMP \| CAP1 \| CAPG \| CAPN1 \| CAPNS1 \| CAPZA1 \| CARHSP1 \| CASP14 \| CAST \| CAT \| CBS \| CCDC126 \| CCDC25 \| CCT3 \| CD109 \| CD276 \| CD48 \| CD5L \| CD9 \| CD93 \| CDA \| CDC5L \| CDH22 \| CDH6 \| CDK2 \| CDSN \| CEACAM8 \| CECR1 \| CENPF \| CEP57 \| CES1 \| CETP \| CFB \| CFHR1 \| CFHR4 \| CFHR5 \| CFI \| CFL1 \| CHGB \| CHIT1 \| CHORDC1 \| CKB \| CKM \| CLIC1 \| CLIC4 \| CLIP1 \| CLPS \| CLTA \| CLTC \| CNN2 \| CNOT1 \| CNTN3 \| COG4 \| COL10A1 \| COL15A1 \| COL19A1 \| COL1A1 \| COL3A1 \| COL4A2 \| COL4A3 \| COL4A6 \| COL5A1 \| COL6A1 \| COL6A2 \| COL9A1 \| COL9A3 \| COLEC10 \| COLEC12 \| COMP \| COPS8 \| CORO1C \| COTL1 \| CPB2 \| CPE \| CPEB3 \| CPM \| CPN1 \| CPNE3 \| CPS1 \| CR1 \| CR2 \| CRHBP \| CRKL \| CRTAC1 \| CRYAA \| CRYBB1 \| CS \| CSRP1 \| CST1 \| CST4 \| CST5 \| CST6 \| CTBS \| CTSA \| CTSC \| CTSG \| CTSH \| CTSZ \| CTTN \| CUX2 \| CYB5A \| CYCS \| CYTL1 \| DAG1 \| DARS2 \| DBH \| DCD \| DCXR \| DDAH1 \| DDT \| DDX3Y \| DEFA6 \| DERP2 \| DGKQ \| DISC1 \| DMGDH \| DMWD \| DNPEP \| DOT1L \| DPEP2 \| DPP3 \| DPYSL2 \| DSC1 \| DSC2 \| DSC3 \| DSCAM \| DSG1 \| DSG2 \| DSP \| DSTN \| ECHS1 \| ECI1 \| ECM1 \| EEF1G \| EEF2 \| EFEMP1 \| EFEMP2 \| EFNB1 \| EFNB2 \| EFTUD2 \| EHD1 \| EHD3 \| EIF3I \| EIF4A1 \| EIF5A \| ELAVL1 \| ELP6 \| EMILIN1 \| EMSY \| EN2 \| ENO1 \| ENO3 \| ENPEP \| ENSA \| ENTPD5 \| EPB41 \| EPHX2 \| EPPK1 \| EPS15 \| EPYC \| ERAP1 \| ERAP2 \| ERP29 \| ESD \| ESR1 \| EVC2 \| EVPL \| EZR \| F11 \| F12 \| F13A1 \| F5 \| F7 \| F9 \| FABP5 \| FAH \| FAM20C \| FAM3C \| FASN \| FASTKD5 \| FBN1 \| FBP1 \| FBP2 \| FBXO38 \| FCGBP \| FCGR2A \| FCGR3A \| FCN2 \| FDPS \| FGB \| FGG \| FGL2 \| FH \| FKBP1A \| FKBP3 \| FKBP5 \| FLG2 \| FLNA \| FLNB \| FLNC \| FOLR3 \| FOXA1 \| FRY \| FSCN1 \| FSHB \| FTL \| FUCA1 \| FUCA2 \| FZD8 \| G0S2 \| G3BP1 \| G6PD \| GALNT2 \| GANAB \| GAPDH \| GART \| GATM \| GBE1 \| GCLC \| GCSH \| GDA \| GDF7 \| GDI1 \| GDI2 \| GFAP \| GGCT \| GGH \| GH2 \| GHRH \| GIP \| GKN1 \| GLIPR2 \| GLRX \| GLUD1 \| GM2A \| GMFB \| GNB1 \| GNPTG \| GOLIM4 \| GOLM1 \| GOT1 \| GOT2 \| GP1BA \| GP5 \| GPD1 \| GPHN \| GPLD1 \| GPT \| GRHL1 \| GRHPR \| GSS \| GSTM1 \| GSTO1 \| GSTP1 \| GUCA2A \| GULP1 \| GZMM \| H2AFZ \| H3F3A \| H6PD \| HABP2 \| HAGH \| HBA1 \| HBB \| HBD \| HBG2 \| HBZ \| HCFC1 \| HDGF \| HEG1 \| HEXB \| HGFAC \| HIBADH \| HINT1 \| HIP1R \| HIST1H1C \| HIST1H1E \| HIST1H2AB \| HIST1H2BK \| HIST1H4A \| HLA-C \| HMGB1 \| HMGB2 \| HMGB3 \| HMGN2 \| HN1 \| HNRNPA1 \| HNRNPA2B1 \| HNRNPC \| HNRNPL \| HNRNPM \| HNRNPU \| HOXB3 \| HOXD11 \| HP1BP3 \| HPD \| HPR \| HPRT1 \| HRG \| HRNR \| HRSP12 \| HSD17B10 \| HSPG2 \| HUWE1 \| ICOSLG \| IGFALS \| ITGA2B \| JUP \| KRT1 \| KRT10 \| KRT13 \| KRT14 \| KRT15 \| KRT16 \| KRT17 \| KRT20 \| KRT3 \| KRT4 \| KRT5 \| KRT9 \| LGALSL \| LPA \| LYPD3 \| MAN1A1 \| MAP3K5 \| MLXIPL \| MYL1 \| NAXE \| NBL1 \| NIF3L1 \| NPPA \| OTUD7B \| PDIA3 \| PDIA4 \| PDLIM3 \| PIP \| PPIA \| PPIB \| PRKCSH \| PROCR \| PROM1 \| PTPRJ \| PVR \| RAD23B \| RALGAPA1 \| RARS \| RBMX \| RNASE2 \| RPSA \| SARNP \| SCG2 \| SERPINC1 \| SERPINH1 \| SFN \| SLC3A2 \| SLC4A1 \| SMARCA4 \| SNCA \| SPTAN1 \| SPTB \| ST13 \| SYNM \| TCERG1 \| TKFC \| TP53BP1 \| TUBA4A \| TUBB1 \| TUBB3 \| UBA1 \| YWHAB \| YWHAE \| YWHAG \| YWHAH \| YWHAQ \| YWHAZ \| ZFHX3
Kit Components	Human Glycosylation Array L3 Glass Slide/s Dilution Buffer20 X Wash Buffer I20 X Wash Buffer IICy3 equivalent dye-conjugated Streptavidin Biotin-labeled Lectin Mixture Adhesive device sealer Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Distilled or de-ionized water 1 ml microcentrifuge tubes Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Incubate samples Incubate with biotin lectin mixture Incubate with Cy3 Equivalent Dye-Streptavidin Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Product Features	High density screening High sensitivity and specificity Low sample volume (20-100 µl per array) Large dynamic range of detection Compatible with most sample types Accurate and reproducible
UNSPSC Code	41116133
Introduction	Recent technological advances by RayBiotech have enabled the largest commercially available glycosylation antibody array to date. With the Human Glycosylation Array L3, researchers can now efficiently obtain glycosylation profiles in their samples. The glycosylation profiles of 500 human proteins can be simultaneously detected, including, but not limited to, cytokines, chemokines, adipokines, growth factors, angiogenic factors, proteases, soluble receptors, soluble adhesion molecules in cell culture supernatants, serum, plasma and other samples types.Capture antibodies are printed onto glass slides and the glycans on these capture antibodies are removed. The glass slide arrays come pre-blocked and are ready to be incubated with samples. After incubation with samples and washing to remove unbound proteins, five unique biotin-labeled lectins are incubated with the array to allow binding to the glycans from captured target proteins. Streptavidin-conjugated fluorescent dye (Cy3 equivalent) is then added to the array. Finally, the glass slide is dried and laser fluorescence scanning is used to visualize the signals. These signals are then compared to the array map to identify glycosylated proteins present in the samples.
Typical Results	The following figure shows the RayBio® Human Glycosylation Antibody Array L3 probed with conditioned media sample. The images were captured using a Axon GenePix laser scanner. The strong signals in row 20 and the upper left and lower right corners of each array are Positive Controls, which can be used to identify the orientation and help normalize the results between arrays.
Biotin-Labeled Lectin Mixture	Lectin Name Sugar specificity

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