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Anti-Metabotropic Glutamate Receptor Type 1 (mGluR1) Antibody

ArtNr ER1
Hersteller Aves Labs
Menge 1000 uL
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen IHC, ICC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Chicken
Isotype IgY
Konjugat/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Metabotropic glutamate receptor 1, mGluR1
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibody
Manufacturer - Category
Neurotransmitter Receptors
Manufacturer - Targets
Metabotropic Glutamate Receptor Type 1 (mGluR1)
Country of Origin
United States
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Molecular Weight
130 kDa
Concentration
300 µg/mL
Buffer
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality Control
This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Recommended Dilutions
1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Volume
Regular vials contain 1000 µ, l of this antibody mixture, Sampler vials contain 200 µ, l of this mixture.
Description
We have three different anti-mGluR antibodies, each of which binds to a specific peptide sequence(s) present in different sets of mGluR gene family members. The ER1 antibody represents sequences found only in mGluR1. It comprises three different antipeptide antibodies generated against three different regions of the mGluR1 gene product not found in the other mGluR gene family members. This antibody has been validated for immunohistochemistry and immunocytochemistry using mouse brain sections and cultured neuroblastoma cells as well as in rat brain sections.
Manufacturer - Statement
We produce our Anti-Metabotropic Glutamate Receptor Type 1 (mGluR1) chicken polyclonal primary antibody. It is great in IHC, ICC and is affinity-purified IgY.
Target Description
The metabotropic glutamate receptors (mGluRs) represent a gene family of G-protein-coupled receptors, all of which bind the amino acid glutamate (the major excitatory neurotransmitter of the nervous system) but differ in their primary amino acid structure, their pharmacology, and their distribution in the peripheral and central nervous systems. In general, mGluR1 and mGluR5 receptors are post-synaptic and mGluR2 and mGluR3 receptors are pre-synaptic.
Antibody Registry ID (RRID)
AB_2313541
Clonality
Polyclonal
Dilution Range ICC
1:1000-1:2000
Dilution Range IHC
1:1000-1:2000
Immunogen
Synthetic peptides
Immunogen Species
Human, Mouse, Rat
Manufacturer - Form
Affinity-Purified IgY
Expiration
12 months from receipt of product
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes
Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 1 gene product, but are shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1000 uL
Lieferbar: In stock
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