Vergleich

Anti-Netrin-1 Antibody

ArtNr NET
Hersteller Aves Labs
Menge 1000 uL
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen IHC, ICC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Chicken
Isotype IgY
Konjugat/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Netrin-1, Epididymis tissue protein Li 131P
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibody
Manufacturer - Category
Cell Markers, Neuronal Markers
Manufacturer - Targets
Netrin-1
Country of Origin
United States
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Molecular Weight
67 kDa
Concentration
100 µg/mL
Buffer
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality Control
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Recommended Dilutions
1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Volume
Regular vials contain 1000 µ, l of this antibody mixture, Sampler vials contain 200 µ, l of this mixture.
Description
An antipeptide antibody was generated in chickens against a sequence shared between the mouse (O09118) and human (O95631) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL. These antibodies have been validated with human, mouse and rat tissues.
Manufacturer - Statement
We produce our Anti-Netrin-1 chicken polyclonal primary antibody. It is great in IHC, ICC and is affinity-purified IgY.
Target Description
Murine netrin-1 precursor is a 67, 768 dalton protein (604 amino acids) expressed by subpopulations of cells within the developing CNS, as well as in various non-neural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of "commissural neurons" from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS.
Antibody Registry ID (RRID)
AB_2313551
Clonality
Polyclonal
Dilution Range ICC
1:1000-1:2000
Dilution Range IHC
1:1000-1:2000
Dilution Range Western Blot
Not Recommended
Dilution Range Western Blot Brain
Not Recommended
Immunogen
Synthetic peptide
Immunogen Species
Human, Mouse, Rat
Manufacturer - Form
Affinity-Purified IgY
Expiration
12 months from receipt of product
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes
Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the netrin-1 gene product, but was shared between the human (O_095631, NCBI) and mouse (O_09118, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation filter-sterilized.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1000 uL
Lieferbar: In stock
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