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Netrin-1

Netrin-1

Manufacturer	Aves Labs
Category	Neuroscience Molecular Targets for Neuroscience Immunohistochemistry Immunocytochemistry Cell Lines & Cell Culture Cell Lines
Type	Neuron Cell Marker
Specific against	Human, Mouse
Applications	IHC, ICC
Amount	1000 ul concentration: 100 ug/ml
Item no.	NET
Targets	NTN1
eClass 6.1	32160890
eClass 9.0	32160890
Available
Buffer	Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Description	Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the netrin-1 gene product, but was shared between the human (O_095631, NCBI) and mouse (O_09118, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.
Quality Control	This antibody preparation was analyzed by immunohistochemistry (at a concentration of 3 µ, g/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Recommended Dilutions	1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Volume	Regular vials contain 1000 µ, l of this antibody mixture, Sampler vials contain 200 µ, l of this mixture.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1000 ul concentration: 100 ug/ml

available

Delivery expected until 6/13/2024