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uPA ELISA Kit (Human)

uPA ELISA Kit (Human)

ArtNr

OKBB00267

Hersteller

AVIVA Systems Biology

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Human (Homo sapiens)

Sensitivity

<5 pg/ml

Citations

1. Finckh, U.; van Hadeln, K.; Muller-Thomsen, T.; Alberici, A.; Binetti, G.; Hock, C.; Nitsch, R. M.; Stoppe, G.; Reiss, J.;
Gal, A. : Association of late-onset Alzheimer disease with a genotype of PLAU, the gene encoding urokinase-type
plasminogen activator on chromosome 10q22.2. Neurogenetics 4: 213-217, 2003.
2. Kiian, I.; Tkachuk, N.; Haller, H.; Dumler, I. : Urokinase-induced migration of human vascular smooth muscle cells
requires coupling of the small GTPase RhoA and Rac1 to the Tyk2/PI3-K signalling pathway. Thromb. Haemost. 89:
904-914, 2003.

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

ATF,BDPLT5,Plasminogen activator urinary,PLAU,QPD,u PA,U-plasminogen activator,uPA,URK,UROK_HUMAN,Urokinase-type plasminogen activator chain B

Versandbedingung

Gekühlt

Lieferbar

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/ELISA Kits, Root Catalog/Products/ELISA Kits/Cell Adhesion

Shipping Temperature

Wet Ice

Gene symbol

PLAU

Gene Fullname

plasminogen activator, urokinase

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Plasminogen activator, urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).

Nucleotide accession_num

NM_001145031.2

Protein accession_num

NP_001138503.1

Protein name

Urokinase-type plasminogen activator

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-L431

Manufacturer - Specificity

Natural and recombinant human Upa

Kit duration

~ 3 Hours

Kit principle

Aviva's human uPA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for uPA has been precoated onto 96-well plates. Standards(NS0, M1-L431) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human uPA amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, serum and plasma(heparin, EDTA)

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKBB00267.pdf

OKBB00267-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 96 wells/kit, with removable strips.

lieferbar

Lieferung vsl. bis 30.10.2025

Express-Lieferung: 8 Tage sparen

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