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uPA ELISA Kit (Human)

uPA ELISA Kit (Human)

Item no.

OKBB00267

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Sensitivity

<5 pg/ml

Citations

1. Finckh, U.; van Hadeln, K.; Muller-Thomsen, T.; Alberici, A.; Binetti, G.; Hock, C.; Nitsch, R. M.; Stoppe, G.; Reiss, J.;
Gal, A. : Association of late-onset Alzheimer disease with a genotype of PLAU, the gene encoding urokinase-type
plasminogen activator on chromosome 10q22.2. Neurogenetics 4: 213-217, 2003.
2. Kiian, I.; Tkachuk, N.; Haller, H.; Dumler, I. : Urokinase-induced migration of human vascular smooth muscle cells
requires coupling of the small GTPase RhoA and Rac1 to the Tyk2/PI3-K signalling pathway. Thromb. Haemost. 89:
904-914, 2003.

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

ATF,BDPLT5,Plasminogen activator urinary,PLAU,QPD,u PA,U-plasminogen activator,uPA,URK,UROK_HUMAN,Urokinase-type plasminogen activator chain B

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/ELISA Kits, Root Catalog/Products/ELISA Kits/Cell Adhesion

Shipping Temperature

Wet Ice

Gene symbol

PLAU

Gene Fullname

plasminogen activator, urokinase

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Plasminogen activator, urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).

Nucleotide accession_num

NM_001145031.2

Protein accession_num

NP_001138503.1

Protein name

Urokinase-type plasminogen activator

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-L431

Manufacturer - Specificity

Natural and recombinant human Upa

Kit duration

~ 3 Hours

Kit principle

Aviva's human uPA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for uPA has been precoated onto 96-well plates. Standards(NS0, M1-L431) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human uPA amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, serum and plasma(heparin, EDTA)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB00267.pdf

OKBB00267-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 10/30/2025

Express Delivery: Save 8 days

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