Tetracyclines ELISA Kit

ArtNr	OKAO00129
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neural Lineage Markers By Cell Type Blastoma Carcinoma Cell Biology Cell Status ELISA Proliferation ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer
Typ	Elisa-Kit
Specific against	other
Sensitivity	<table class='tbl-grid'><tr><th>Sample</th><th>Amount</th></tr><tr><td>Honey</td><td>1 ppb</td></tr><tr><td>Milk</td><td>2 ppb</td></tr><tr><td>Tissue, Egg</td><td>2 ppb</td></tr><tr><td>Water</td><td>0.5 ppb</td></tr></table>
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	TET,Tetracycline,Deschlorobiomycin,Tetracyclinum,Achromycin,60-54-8,Tetracyclin
Versandbedingung	Gekühlt
Lieferbar
Specificity	General
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kit
Shipping Temperature	Wet Ice
Molecular Weight	444.43456 g/mol
Reconstitution and storage	Store as indicated in product manual.
Description of target	Tetracycline is a broad-spectrum naphthacene antibiotic produced semisynthetically from chlortetracycline, an antibiotic isolated from the bacterium Streptomyces aureofaciens. In bacteria, tetracycline binds to the 30S ribosomal subunit, interferes with the binding of aminoacyl-tRNA to the mRNA-ribosome complex, thereby inhibiting protein synthesis.
Protein name	Tetracyclines
Manufacturer - Specificity	Substance \| Cross Reactivity Rate Tetracycline \| 100% Doxycycline \| 120% Aureomycin \| 130% Oxytetracycline \| 120%
Assay info	Assay Methodology: Competitive Inhibition ELISA
Kit duration	~4 Hours
Kit principle	Aviva Systems Biology Tetracyclines ELISA Kit (OKAO00129) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Tetracyclines has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Tetracyclines antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
Kit recovery	Sample - Amount Honey, milk - 90% +/- 10%Tissue, egg - 90% +/- 15% Water - 80% +/- 10%
Kit reproducibility	Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40)
Sample Type	Tissue (Including aquatic products), Honey, Milk, Egg, Water.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKAO00129.pdf

OKAO00129-datasheet.pdf

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Menge: 96 Wells

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