Comparison

Tetracyclines ELISA Kit

Item no. OKAO00129
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Specific against other
Sensitivity <table class='tbl-grid'><tr><th>Sample</th><th>Amount</th></tr><tr><td>Honey</td><td>1 ppb</td></tr><tr><td>Milk</td><td>2 ppb</td></tr><tr><td>Tissue, Egg</td><td>2 ppb</td></tr><tr><td>Water</td><td>0.5 ppb</td></tr></table>
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias TET,Tetracycline,Deschlorobiomycin,Tetracyclinum,Achromycin,60-54-8,Tetracyclin
Shipping Condition Cool pack
Available
Specificity General
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kit
Shipping Temperature
Wet Ice
Molecular Weight
444.43456 g/mol
Reconstitution and storage
Store as indicated in product manual.
Description of target
Tetracycline is a broad-spectrum naphthacene antibiotic produced semisynthetically from chlortetracycline, an antibiotic isolated from the bacterium Streptomyces aureofaciens. In bacteria, tetracycline binds to the 30S ribosomal subunit, interferes with the binding of aminoacyl-tRNA to the mRNA-ribosome complex, thereby inhibiting protein synthesis.
Protein name
Tetracyclines
Manufacturer - Specificity
Substance | Cross Reactivity Rate
Tetracycline | 100%
Doxycycline | 120%
Aureomycin | 130%
Oxytetracycline | 120%
Assay info
Assay Methodology: Competitive Inhibition ELISA
Kit duration
~4 Hours
Kit principle
Aviva Systems Biology Tetracyclines ELISA Kit (OKAO00129) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Tetracyclines has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Tetracyclines antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
Kit recovery
Sample - Amount
Honey, milk - 90% +/- 10%Tissue, egg - 90% +/- 15%
Water - 80% +/- 10%
Kit reproducibility
Mean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
Sample Type
Tissue (Including aquatic products), Honey, Milk, Egg, Water.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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