Fumonisin B1 ELISA Kit

ArtNr	OKAO00145
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Food Safety Mycotoxins ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	other
Sensitivity	<table class='tbl-grid'><tr><th>Sample</th><th>Amount</th></tr><tr><td>Corn, Feed</td><td>50 ppb</td></tr><tr><td>Cooking oil</td><td>10 ppb</td></tr></table>
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	FB1,Fumonisin b1,Macrofusine,Fumonisin B1,Fusarium moniliforme,NSC629151,116355-83-0,FB1
Versandbedingung	Gekühlt
Lieferbar
Specificity	General
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kit
Shipping Temperature	Wet Ice
Molecular Weight	721.82996 g/mol
Reconstitution and storage	Store as indicated in product manual.
Description of target	Fumonisin B1 is the most prevalent member of a family of toxins, known as fumonisins, produced by several species of Fusarium molds, such as Fusarium verticillioides, [1] which occur mainly in maize (corn), wheat and other cereals. Fumonisin B1 contamination of maize has been reported worldwide at mg/kg levels. Human exposure occurs at levels of micrograms to milligrams per day and is greatest in regions where maize products are the dietary staple.
Protein name	Fumonisin B1
Manufacturer - Specificity	Substance \| Cross Reactivity Rate Fumonisin B1 \| 100%
Assay info	Assay Methodology: Competitive Inhibition ELISA
Kit duration	~4 Hours
Kit principle	Aviva Systems Biology Fumonisin B1 ELISA Kit (OKAO00145) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Fumonisin B1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Fumonisin B1 antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
Kit recovery	Sample - Amount Corn - 100% +/- 15% Feed - 95% +/- 15%Cooking oil - 85% +/- 15%
Kit reproducibility	Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40)
Sample Type	Corn, feed and cooking oil

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKAO00145.pdf

OKAO00145-datasheet.pdf

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Menge: 96 Wells

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