Fumonisin B1 ELISA Kit

ELISA Kit

Wet Ice

Store as indicated in product manual.

Fumonisin B1

Assay Methodology: Competitive Inhibition ELISA

Aviva Systems Biology Fumonisin B1 ELISA Kit (OKAO00145) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Fumonisin B1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Fumonisin B1 antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.

Mean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)