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NOV ELISA Kit (Human)

ArtNr OKBB00827
Hersteller AVIVA Systems Biology
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
Konjugat/Tag Biotin
Sensitivity <2 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias ccn 3,CCN family member 3,CCN3,IGFBP9,NOVH,IBP-9,IGF-binding protein 9,IGFBP9,IGFBP-9,nephroblastoma overexpressed,NovH,P48745,Protein NOV homolog
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
CCN3
Gene Fullname
cellular communication network factor 3
Reconstitution and storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Description of target
NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.
Nucleotide accession_num
NM_002514.3
Protein accession_num
NP_002505.1
Protein name
CCN family member 3
Immunogen
Expression system for standard: NS0; Immunogen sequence: T32-M357
Manufacturer - Specificity
Natural and recombinant human CCN3
Kit duration
~ 3 Hours
Kit principle
Aviva's human CCN3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CCN3 has been precoated onto 96-well plates. Standards(NS0, T32-M357) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCN3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCN3 amount of sample captured in plate.
Kit reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample
Sample Type
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

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Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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