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NOV ELISA Kit (Human)

NOV ELISA Kit (Human)

Item no.

OKBB00827

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Conjugate/Tag

Biotin

Sensitivity

<2 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

ccn 3,CCN family member 3,CCN3,IGFBP9,NOVH,IBP-9,IGF-binding protein 9,IGFBP9,IGFBP-9,nephroblastoma overexpressed,NovH,P48745,Protein NOV homolog

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

CCN3

Gene Fullname

cellular communication network factor 3

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.

Nucleotide accession_num

NM_002514.3

Protein accession_num

NP_002505.1

Protein name

CCN family member 3

Immunogen

Expression system for standard: NS0; Immunogen sequence: T32-M357

Manufacturer - Specificity

Natural and recombinant human CCN3

Kit duration

~ 3 Hours

Kit principle

Aviva's human CCN3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CCN3 has been precoated onto 96-well plates. Standards(NS0, T32-M357) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCN3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCN3 amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB00827.pdf

OKBB00827-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 1/8/2026

Express Delivery: Save 8 days

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