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FGF23 ELISA Kit (Mouse)

ArtNr OKBB00944
Hersteller AVIVA Systems Biology
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Konjugat/Tag Biotin
Sensitivity <10 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias ADHR,FGF-23,Fgf23,FGF23_HUMAN,FGFN,Fibroblast growth factor 23,Fibroblast growth factor 23 C-terminal peptide,Fibroblast growth factor 23 precursor,HPDR2,HYPF,Phosphatonin,PHPTC,Tumor derived hypophosphatemia inducing factor,Tumor-derived hypophosphatemia-inducing factor
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
FGF23
Gene Fullname
fibroblast growth factor 23
Reconstitution and storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Description of target
Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.
Nucleotide accession_num
NM_022657.4
Protein accession_num
NP_073148.1
Protein name
Fibroblast growth factor 23
Immunogen
Expression system for standard: E.coli; Immunogen sequence: Y25-V251
Manufacturer - Specificity
Natural and recombinant mouse FGF23
Kit duration
~ 3 Hours
Kit principle
Aviva's mouse FGF23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for FGF23 has been precoated onto 96-well plates. Standards(E.coli, Y25-V251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse FGF23 amount of sample captured in plate.
Kit reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample
Sample Type
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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