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FGF23 ELISA Kit (Mouse)

FGF23 ELISA Kit (Mouse)

Item no.

OKBB00944

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Mouse (Murine, Mus musculus)

Conjugate/Tag

Biotin

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

ADHR,FGF-23,Fgf23,FGF23_HUMAN,FGFN,Fibroblast growth factor 23,Fibroblast growth factor 23 C-terminal peptide,Fibroblast growth factor 23 precursor,HPDR2,HYPF,Phosphatonin,PHPTC,Tumor derived hypophosphatemia inducing factor,Tumor-derived hypophosphatemia-inducing factor

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

FGF23

Gene Fullname

fibroblast growth factor 23

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.

Nucleotide accession_num

NM_022657.4

Protein accession_num

NP_073148.1

Protein name

Fibroblast growth factor 23

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Y25-V251

Manufacturer - Specificity

Natural and recombinant mouse FGF23

Kit duration

~ 3 Hours

Kit principle

Aviva's mouse FGF23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for FGF23 has been precoated onto 96-well plates. Standards(E.coli, Y25-V251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse FGF23 amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB00944.pdf

OKBB00944-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 12/25/2025

Express Delivery: Save 2 days

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