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Apoh ELISA Kit (Mouse)

ArtNr OKBB01395
Hersteller AVIVA Systems Biology
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Konjugat/Tag Biotin
Sensitivity <50 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Beta-2-glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Apolipoprotein H; Apo-H; Beta-2-glycoprotein I; B2GPI; Beta(2)GPI; Apoh; B2gp1
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
APOH
Gene Fullname
apolipoprotein H
Reconstitution and storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Description of target
Apolipoprotein H (Apo-H), previously known as β2-glycoprotein I and beta-2 glycoprotein I, is a 38 kDa multifunctional apolipoprotein that in humans is encoded by the APOH gene. This gene is mapped to 11 E1; 11 71.8 Cm. Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.
Nucleotide accession_num
NM_013475.4
Protein accession_num
NP_038503.4
Protein name
Beta-2-glycoprotein 1
Immunogen
Expression system for standard: NS0; Immunogen sequence: G20-C345
Manufacturer - Specificity
Natural and recombinant mouse APOH
Kit duration
~ 3 Hours
Kit principle
Aviva's mouse APOH ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for APOH has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: G20-C345) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APOH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse APOH amount of sample captured in plate.
Kit reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample
Sample Type
cell culture supernates, serum and plasma (heparin, EDTA)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
lieferbar

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