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Apoh ELISA Kit (Mouse)

Apoh ELISA Kit (Mouse)

Item no.

OKBB01395

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Mouse (Murine, Mus musculus)

Conjugate/Tag

Biotin

Sensitivity

<50 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Beta-2-glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Apolipoprotein H; Apo-H; Beta-2-glycoprotein I; B2GPI; Beta(2)GPI; Apoh; B2gp1

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

APOH

Gene Fullname

apolipoprotein H

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Apolipoprotein H (Apo-H), previously known as Î²2-glycoprotein I and beta-2 glycoprotein I, is a 38 kDa multifunctional apolipoprotein that in humans is encoded by the APOH gene. This gene is mapped to 11 E1; 11 71.8 Cm. Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Nucleotide accession_num

NM_013475.4

Protein accession_num

NP_038503.4

Protein name

Beta-2-glycoprotein 1

Immunogen

Expression system for standard: NS0; Immunogen sequence: G20-C345

Manufacturer - Specificity

Natural and recombinant mouse APOH

Kit duration

~ 3 Hours

Kit principle

Aviva's mouse APOH ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for APOH has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: G20-C345) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APOH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse APOH amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, serum and plasma (heparin, EDTA)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB01395.pdf

OKBB01395-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 12/25/2025

Express Delivery: Save 2 days

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