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Beta-Endorphin ELISA Kit (Rat)

ArtNr OKCD02294
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat (Rattus norvegicus)
Sensitivity 4.62 pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Corticotropin-lipotropin.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Competitive
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
BEP
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Corticotropin: Stimulates the adrenal glands to release cortisol.
Protein name
Pro-opiomelanocortin
Assay info
Assay Methodology: Competitive Inhibition Immunoassay
Kit Components
Component: Amount
Anti-Beta-Endorphin Microplate: 96 Wells (12 x 8 Well Strips)
Beta-Endorphin Lyophilized Standard: 2
Beta-Endorphin Biotin Complex: 1
100X Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Biotin Complex Diluent: 1 x 12 mL
Avidin/HRP Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL
Biotin Complex Reconstitution Buffer: 1 x 300 uL
Kit detection
Colorimetric
Kit duration
2h
Kit linearity
Matrix - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
serum(n=5) - 92-103% - 80-92% - 86-94% - 83-101%
EDTA plasma(n=5) - 88-95% - 79-103% - 80-103% - 94-103%
heparin plasma(n=5) - 79-99% - 95-103% - 98-105% - 99-105%

Kit principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Endorphin (bEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample.
Kit recovery
Matrix - Recovery Range (%) - Average (%)
serum(n=5) - 80-97 - 93
EDTA plasma(n=5) - 82-91 - 87
heparin plasma(n=5) - 85-105 - 88
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 30.10.2025 

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