Beta-Endorphin ELISA Kit (Rat)

Enzyme-linked Immunosorbent assay-Competitive

Wet Ice

2°C to 8°C|-20°C

Pro-opiomelanocortin

Component: Amount
Anti-Beta-Endorphin Microplate: 96 Wells (12 x 8 Well Strips)
Beta-Endorphin Lyophilized Standard: 2
Beta-Endorphin Biotin Complex: 1
100X Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Biotin Complex Diluent: 1 x 12 mL
Avidin/HRP Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL
Biotin Complex Reconstitution Buffer: 1 x 300 uL

2h

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Endorphin (bEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%