« Zurück
Startseite /
AIF1 Chemi-Luminescent ELISA Kit (Human)

AIF1 Chemi-Luminescent ELISA Kit (Human)

ArtNr	OKCD03291
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Typ	Elisa-Kit
Applikationen	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	31.2 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	AIF-1;allograft inflammatory factor 1;IBA1;interferon gamma responsive transcript;ionized calcium-binding adapter molecule 1;IRT1;IRT-1;protein G1.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	AIF1
Gene Fullname	allograft inflammatory factor 1
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Nucleotide accession_num	NM_001318970
Protein accession_num	NP_001305899
Protein name	Allograft inflammatory factor 1
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount AIF1 Microplate: 96 Wells (12 x 8 Well strips) AIF1 Lyophilized Standard: 2 100X AIF1 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Ionized Calcium-binding Adapter Molecule 1 (IBA1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ionized Calcium-binding Adapter Molecule 1 (IBA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ionized Calcium-binding Adapter Molecule 1 (IBA1) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ionized Calcium-binding Adapter Molecule 1 (IBA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ionized Calcium-binding Adapter Molecule 1 (IBA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD03291.pdf

OKCD03291-datasheet.pdf

Sicherheitsdatenblatt anfordern

Einfache Bestellung Periodische Bestellung

Menge: 96 Wells

lieferbar

Lieferung vsl. bis 11.12.2025

Express-Lieferung: 3 Tage sparen

Vergleichen

Auf den Wunschzettel

Angebot anfordern