Vergleich

GDF11 Chemi-Luminescent ELISA Kit (Rat)

ArtNr OKCD03511
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Rat (Rattus norvegicus)
Sensitivity 1.37 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias BMP-11;bone morphogenetic protein 11;GDF-11;growth/differentiation factor 11.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Gdf11
Gene Fullname
growth differentiation factor 11
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. May play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3.
Protein name
Growth/differentiation factor 11
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
Gdf11 Microplate: 96 Wells (12 x 8 Well strips)
Gdf11 Lyophilized Standard: 2
100X Gdf11 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 94-101% - 97-104% - 80-104% - 79-94%
EDTA Plasma (n=5) - 82-99% - 93-103% - 83-102% - 96-103%
Heparin Plasma (n=5) - 79-94% - 90-101% - 81-89% - 91-98%
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 11 (GDF11). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 11 (GDF11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Differentiation Factor 11 (GDF11) level in the sample or standard.
Kit recovery
Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 78-105 - 101
EDTA Plasma (n=5) - 95-104 - 101
Heparin Plasma (n=5) - 92-102 - 99
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 30.10.2025 

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