MBP Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

myelin basic protein

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

NP_001020252

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 98-105% - 96-105% - 95-103% - 94-104%
EDTA Plasma (n=5) - 86-99% - 87-97% - 86-94% - 92-101%
Heparin Plasma (n=5) - 79-95% - 89-103% - 85-101% - 92-99%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 83-101 - 95
EDTA Plasma (n=5) - 78-101 - 94
Heparin Plasma (n=5) - 89-97 - 92

Serum, plasma, cerebrospinal fluid and other biological fluids