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P2RX7 Chemi-Luminescent ELISA Kit (Human)

P2RX7 Chemi-Luminescent ELISA Kit (Human)

ArtNr	OKCD03848
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Typ	Elisa-Kit
Applikationen	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	0.312 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ATP receptor;P2X purinoceptor 7;P2X7;P2X7 receptor;P2Z receptor;Purinergic receptor;purinergic receptor P2X,ligand gated ion channel,7;purinergic receptor P2X7 variant A;purnergic receptor P2X 7.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	P2RX7
Gene Fullname	purinergic receptor P2X 7
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells (PubMed:21821797, PubMed:23303206).
Nucleotide accession_num	NM_002562
Protein accession_num	NP_002553
Protein name	P2X purinoceptor 7
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount P2RX7 Microplate: 96 Wells (12 x 8 Well strips) P2RX7 Lyophilized Standard: 2 100X P2RX7 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD03848.pdf

OKCD03848-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 11.12.2025

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