« Back
Home /
P2RX7 Chemi-Luminescent ELISA Kit (Human)

P2RX7 Chemi-Luminescent ELISA Kit (Human)

Item no.	OKCD03848
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Applications	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	0.312 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ATP receptor;P2X purinoceptor 7;P2X7;P2X7 receptor;P2Z receptor;Purinergic receptor;purinergic receptor P2X,ligand gated ion channel,7;purinergic receptor P2X7 variant A;purnergic receptor P2X 7.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	P2RX7
Gene Fullname	purinergic receptor P2X 7
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells (PubMed:21821797, PubMed:23303206).
Nucleotide accession_num	NM_002562
Protein accession_num	NP_002553
Protein name	P2X purinoceptor 7
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount P2RX7 Microplate: 96 Wells (12 x 8 Well strips) P2RX7 Lyophilized Standard: 2 100X P2RX7 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD03848.pdf

OKCD03848-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 96 Wells

available

Delivery expected until 12/11/2025

Express Delivery: Save 2 days

Compare

Add to wishlist

Get an offer