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HSP70 ELISA Kit (Gallus)

ArtNr OKCD04259
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Chicken (Gallus gallus domesticus)
Sensitivity 0.55 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias dnaK-type molecular chaperone;heat shock 70 kDa protein;heat shock protein 70;HSP70.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
HSPA2
Gene Fullname
heat shock 70kDa protein 2
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release.
Protein name
Heat shock 70 kDa protein
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
Hsp70 Microplate: 96 Wells (12 x 8 Well strips)
Hsp70 Lyophilized Standard: 2
100X Hsp70 HRP-Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL
Kit detection
Colorimetric
Kit duration
3h
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 90-101% - 79-93% - 92-101% - 89-97%
EDTA Plasma (n=5) - 94-102% - 95-103% - 80-92% - 86-103%
Heparin Plasma (n=5) - 96-105% - 79-105% - 92-101% - 88-99%
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70 (HSP70). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 70 (HSP70). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 70 (HSP70), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock Protein 70 (HSP70) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit recovery
Matrix - Recovery range (%) - Average (%)
Serum (n=5) - 98-105 - 102
EDTA Plasma (n=5) - 96-103 - 101
Heparin Plasma (n=5) - 79-105 - 81
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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Lieferung vsl. bis 30.10.2025 

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