HSP70 ELISA Kit (Gallus)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

heat shock 70kDa protein 2

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release.

Assay Methodology: Quantitative Sandwich ELISA

Colorimetric

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 90-101% - 79-93% - 92-101% - 89-97%
EDTA Plasma (n=5) - 94-102% - 95-103% - 80-92% - 86-103%
Heparin Plasma (n=5) - 96-105% - 79-105% - 92-101% - 88-99%

Matrix - Recovery range (%) - Average (%)
Serum (n=5) - 98-105 - 102
EDTA Plasma (n=5) - 96-103 - 101
Heparin Plasma (n=5) - 79-105 - 81

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids