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PRKAB1 ELISA Kit (Human)

PRKAB1 ELISA Kit (Human)

ArtNr	OKCD07572
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism By Cell Type Hematology Lymphoma Cell Biology Obesity Cell Status ELISA Autophagy ELISA & Immunoassays ELISA Kits Immunoassay By Organ Pancreatic Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.124ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	5'-AMP-activated protein kinase beta-1 subunit;5'-AMP-activated protein kinase subunit beta-1;AMP-activated protein kinase beta subunit;AMPK;AMPK beta 1;AMPK beta -1 chain;AMPK subunit beta-1;AMPKb;HAMPKb;protein kinase,AMP-activated,beta 1 non-catalytic subunit;protein kinase,AMP-activated,noncatalytic,beta-1.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	PRKAB1
Gene Fullname	protein kinase AMP-activated non-catalytic subunit beta 1
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Nucleotide accession_num	NM_006253.4
Protein accession_num	NP_006244.2
Protein name	5'-AMP-activated protein kinase subunit beta-1
Kit Components	Component: Amount PRKAB1 Microplate: 96 Wells (12 x 8 Well strips)PRKAB1 Lyophilized Standard: 2100X PRKAB1 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Beta 1 (PRKAb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Kinase, AMP Activated Beta 1 (PRKAb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, AMP Activated Beta 1 (PRKAb1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, AMP Activated Beta 1 (PRKAb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids.

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OKCD07572.pdf

OKCD07572-datasheet.pdf

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Menge: 96 Wells

lieferbar

Lieferung vsl. bis 30.10.2025