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PRKAB1 ELISA Kit (Human)

PRKAB1 ELISA Kit (Human)

Item no.	OKCD07572
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism By Cell Type Hematology Lymphoma Cell Biology Obesity Cell Status ELISA Autophagy ELISA & Immunoassays ELISA Kits Immunoassay By Organ Pancreatic Cancer Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.124ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	5'-AMP-activated protein kinase beta-1 subunit;5'-AMP-activated protein kinase subunit beta-1;AMP-activated protein kinase beta subunit;AMPK;AMPK beta 1;AMPK beta -1 chain;AMPK subunit beta-1;AMPKb;HAMPKb;protein kinase,AMP-activated,beta 1 non-catalytic subunit;protein kinase,AMP-activated,noncatalytic,beta-1.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	PRKAB1
Gene Fullname	protein kinase AMP-activated non-catalytic subunit beta 1
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Nucleotide accession_num	NM_006253.4
Protein accession_num	NP_006244.2
Protein name	5'-AMP-activated protein kinase subunit beta-1
Kit Components	Component: Amount PRKAB1 Microplate: 96 Wells (12 x 8 Well strips)PRKAB1 Lyophilized Standard: 2100X PRKAB1 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Beta 1 (PRKAb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Kinase, AMP Activated Beta 1 (PRKAb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, AMP Activated Beta 1 (PRKAb1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, AMP Activated Beta 1 (PRKAb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD07572.pdf

OKCD07572-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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