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CPA4 ELISA Kit (Human)

CPA4 ELISA Kit (Human)

ArtNr	OKDD00203
Hersteller	AVIVA Systems Biology
Menge	96 tests
Kategorie	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Adenocarcinoma Mesothelioma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.051 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	carboxypeptidase A3;carboxypeptidase A4;CPA3.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Molecular Weight	47 kDa
Gene symbol	CPA4
Gene Fullname	carboxypeptidase A4
Protein size	421
Reconstitution and storage	Store as indicated in product manual.
Description of target	Metalloprotease that could be involved in the histone hyperacetylation pathway (PubMed:10383164). Releases a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val (PubMed:20385563).
Nucleotide accession_num	NM_001163446.1
Protein accession_num	NP_001156918.1
Protein name	Carboxypeptidase A4
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of CPA4.No significant cross-reactivity or interference between CPA4 and analogues was observed.
Assay info	Quantitative Sandwich ELISA
Kit duration	~4 Hours
Kit principle	Aviva Systems Biology CPA4 ELISA Kit (Human) (OKDD00203) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for CPA4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPA4 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CPA4 captured in well.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CPA4 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CPA4 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKDD00203.pdf

OKDD00203-datasheet.pdf

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Menge: 96 tests

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Lieferung vsl. bis 25.12.2025

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