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CPA4 ELISA Kit (Human)

CPA4 ELISA Kit (Human)

Item no.	OKDD00203
Manufacturer	AVIVA Systems Biology
Amount	96 tests
Category	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Adenocarcinoma Mesothelioma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.051 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	carboxypeptidase A3;carboxypeptidase A4;CPA3.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Molecular Weight	47 kDa
Gene symbol	CPA4
Gene Fullname	carboxypeptidase A4
Protein size	421
Reconstitution and storage	Store as indicated in product manual.
Description of target	Metalloprotease that could be involved in the histone hyperacetylation pathway (PubMed:10383164). Releases a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val (PubMed:20385563).
Nucleotide accession_num	NM_001163446.1
Protein accession_num	NP_001156918.1
Protein name	Carboxypeptidase A4
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of CPA4.No significant cross-reactivity or interference between CPA4 and analogues was observed.
Assay info	Quantitative Sandwich ELISA
Kit duration	~4 Hours
Kit principle	Aviva Systems Biology CPA4 ELISA Kit (Human) (OKDD00203) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for CPA4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPA4 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CPA4 captured in well.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CPA4 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CPA4 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKDD00203.pdf

OKDD00203-datasheet.pdf

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Amount: 96 tests

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Delivery expected until 12/25/2025

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