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LY96 ELISA Kit (Human)

ArtNr OKDD00383
Hersteller AVIVA Systems Biology
Menge 96 tests
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
Sensitivity < 0.06 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias ESOP-1;ly-96;lymphocyte antigen 96;MD2;MD-2;myeloid differentiation protein-2;protein MD-2.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Molecular Weight
19 kDa
Gene symbol
LY96
Gene Fullname
lymphocyte antigen 96
Protein size
160
Reconstitution and storage
Store as indicated in product manual.
Description of target
Binds bacterial lipopolysaccharide (LPS) (PubMed:17803912, PubMed:17569869). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria (PubMed:11160242, PubMed:11593030). Enhances TLR4-dependent activation of NF-kappa-B (PubMed:10359581). Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10359581).
Nucleotide accession_num
NM_001195797.1
Protein accession_num
NP_001182726.1
Protein name
Lymphocyte antigen 96
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of LY96.No significant cross-reactivity or interference between LY96 and analogues was observed.
Assay info
Quantitative Sandwich ELISA
Kit duration
~4 Hours
Kit principle
Aviva Systems Biology LY96 ELISA Kit (Human) (OKDD00383) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for LY96 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LY96 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LY96 captured in well.
Kit reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LY96 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LY96 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Sample Type
Serum, plasma, tissue homogenates, cell lysates

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 tests
Lieferbar: In stock
lieferbar

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