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LY96 ELISA Kit (Human)

LY96 ELISA Kit (Human)

Item no.	OKDD00383
Manufacturer	AVIVA Systems Biology
Amount	96 tests
Category	Neuroscience Neural Lineage Markers ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.06 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ESOP-1;ly-96;lymphocyte antigen 96;MD2;MD-2;myeloid differentiation protein-2;protein MD-2.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Molecular Weight	19 kDa
Gene symbol	LY96
Gene Fullname	lymphocyte antigen 96
Protein size	160
Reconstitution and storage	Store as indicated in product manual.
Description of target	Binds bacterial lipopolysaccharide (LPS) (PubMed:17803912, PubMed:17569869). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria (PubMed:11160242, PubMed:11593030). Enhances TLR4-dependent activation of NF-kappa-B (PubMed:10359581). Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10359581).
Nucleotide accession_num	NM_001195797.1
Protein accession_num	NP_001182726.1
Protein name	Lymphocyte antigen 96
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of LY96.No significant cross-reactivity or interference between LY96 and analogues was observed.
Assay info	Quantitative Sandwich ELISA
Kit duration	~4 Hours
Kit principle	Aviva Systems Biology LY96 ELISA Kit (Human) (OKDD00383) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for LY96 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LY96 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LY96 captured in well.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LY96 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LY96 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 tests

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Delivery expected until 12/25/2025

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