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DCX ELISA Kit (Mouse)

ArtNr OKDD00730
Hersteller AVIVA Systems Biology
Menge 96 tests
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Sensitivity <0.051 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Dbct;doublecortex;doublin;lissencephalin-X;lissencephaly,X-linked (doublecortin);lis-X;neuronal migration protein doublecortin.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Molecular Weight
41 kDa
Gene symbol
Dcx
Gene Fullname
doublecortin
Protein size
366
Reconstitution and storage
Store as indicated in product manual.
Description of target
Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May participate along with PAFAH1B1/LIS-1 in a distinct overlapping signaling pathway that promotes neuronal migration.
Nucleotide accession_num
NM_001110222.1
Protein accession_num
NP_001103692.1
Protein name
Neuronal migration protein doublecortin
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of DCX.No significant cross-reactivity or interference between DCX and analogues was observed.
Assay info
Quantitative Sandwich ELISA
Kit duration
~4 Hours
Kit principle
Aviva Systems Biology DCX ELISA Kit (Mouse) (OKDD00730) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for DCX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DCX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DCX captured in well.
Kit reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

Sample Type
Serum, plasma, tissue homogenates, cell lysates

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 tests
Lieferbar: In stock
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