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DCX ELISA Kit (Mouse)

DCX ELISA Kit (Mouse)

Item no.	OKDD00730
Manufacturer	AVIVA Systems Biology
Amount	96 tests
Category	Neuroscience Metabolism Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience ELISA Enzymes Fatty Acid Catabolism ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<0.051 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Dbct;doublecortex;doublin;lissencephalin-X;lissencephaly,X-linked (doublecortin);lis-X;neuronal migration protein doublecortin.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Molecular Weight	41 kDa
Gene symbol	Dcx
Gene Fullname	doublecortin
Protein size	366
Reconstitution and storage	Store as indicated in product manual.
Description of target	Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May participate along with PAFAH1B1/LIS-1 in a distinct overlapping signaling pathway that promotes neuronal migration.
Nucleotide accession_num	NM_001110222.1
Protein accession_num	NP_001103692.1
Protein name	Neuronal migration protein doublecortin
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of DCX.No significant cross-reactivity or interference between DCX and analogues was observed.
Assay info	Quantitative Sandwich ELISA
Kit duration	~4 Hours
Kit principle	Aviva Systems Biology DCX ELISA Kit (Mouse) (OKDD00730) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for DCX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DCX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DCX captured in well.
Kit reproducibility	Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV<10% Mean Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKDD00730.pdf

OKDD00730-datasheet.pdf

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Amount: 96 tests

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Delivery expected until 11/13/2025

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