FTO ELISA Kit (Rat)

ArtNr	OKEH03710
Hersteller	AVIVA Systems Biology
Menge	96 tests
Kategorie	Metabolism Diabetes Glucose Homeostasis Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	0.086 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	alpha-ketoglutarate-dependent dioxygenase FTO;fat mass and obesity associated;fat mass and obesity-associated protein;m6A(m)-demethylase FTO;mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO;mRNA N(6)-methyladenosine demethylase FTO;protein fto;RGD1305121;tRNA N1-methyl adenine demethylase FTO;U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO;U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Molecular Weight	58 kDa
Gene symbol	Fto
Gene Fullname	FTO, alpha-ketoglutarate dependent dioxygenase
Protein size	502
Reconstitution and storage	Store as indicated in product manual.
Description of target	Alpha-ketoglutarate-dependent dioxygenase FTO
Nucleotide accession_num	NM_001039713.1
Protein accession_num	NP_001034802.1
Protein name	Q2A121
Manufacturer - Specificity	Natural and recombinant Rat Alpha-ketoglutarate-dependent dioxygenase FTO
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit duration	~ 3 Hours
Kit principle	Aviva Systems Biology FTO ELISA Kit (Rat) (OKEH03710) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fto has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fto is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fto captured in well.
Sample Type	Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

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OKEH03710.pdf

OKEH03710-datasheet.pdf

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