GAPDH ELISA Kit (Bovine)

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GAPDH

Store as indicated in product manual.

P10096

Assay Methodology: Lot dependent. Can either be Quantitative or Sandwich ELISA.

Aviva Systems Biology GAPDH ELISA Kit (Bovine) (OKEH06338) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GAPDH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GAPDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GAPDH captured in the well.

Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 4.5% (n = 20)
Mean Inter-Assay Precision - <= 9.7% (n = 20)