Comparison

GAPDH ELISA Kit (Bovine)

Item no. OKEH06338
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications ELISA
Specific against Cow (Bos taurus), Cattle (Bovine)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias GAPD;glyceraldehyde phosphate dehydrogenase;glyceraldehyde-3-phosphate dehydrogenase;peptidyl-cysteine S-nitrosylase GAPDH.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
GAPDH
Gene Fullname
glyceraldehyde-3-phosphate dehydrogenase
Reconstitution and storage
Store as indicated in product manual.
Description of target
Glyceraldehyde-3-phosphate dehydrogenase
Protein name
P10096
Manufacturer - Specificity
Natural and recombinant Bovine Glyceraldehyde-3-phosphate dehydrogenase
Assay info
Assay Methodology: Lot dependent. Can either be Quantitative or Sandwich ELISA.
Kit duration
~3 Hours
Kit principle
Aviva Systems Biology GAPDH ELISA Kit (Bovine) (OKEH06338) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GAPDH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GAPDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GAPDH captured in the well.
Kit recovery
Mean recovery when spiking into Serum and Plasma = 95%
Kit reproducibility
Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 4.5% (n = 20)
Mean Inter-Assay Precision - <= 9.7% (n = 20)
Sample Type
Serum, plasma and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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